5z58: Difference between revisions

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'''Unreleased structure'''


The entry 5z58 is ON HOLD
==Cryo-EM structure of a human activated spliceosome (early Bact) at 4.9 angstrom.==
<SX load='5z58' size='340' side='right' viewer='molstar' caption='[[5z58]], [[Resolution|resolution]] 4.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5z58]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z58 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z58 OCA], [https://pdbe.org/5z58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z58 RCSB], [https://www.ebi.ac.uk/pdbsum/5z58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z58 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CWC15_HUMAN CWC15_HUMAN] Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.<ref>PMID:20176811</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
During each cycle of pre-mRNA splicing, the pre-catalytic spliceosome (B complex) is converted into the activated spliceosome (B(act) complex), which has a well-formed active site but cannot proceed to the branching reaction. Here, we present the cryo-EM structure of the human B(act) complex in three distinct conformational states. The EM map allows atomic modeling of nearly all protein components of the U2 small nuclear ribonucleoprotein (snRNP), including three of the SF3a complex and seven of the SF3b complex. The structure of the human B(act) complex contains 52 proteins, U2, U5, and U6 small nuclear RNA (snRNA), and a pre-mRNA. Three distinct conformations have been captured, representing the early, mature, and late states of the human B(act) complex. These complexes differ in the orientation of the Switch loop of Prp8, the splicing factors RNF113A and NY-CO-10, and most components of the NineTeen complex (NTC) and the NTC-related complex. Analysis of these three complexes and comparison with the B and C complexes reveal an ordered flux of components in the B-to-B(act) and the B(act)-to-B(*) transitions, which ultimately prime the active site for the branching reaction.


Authors:  
Structure of the human activated spliceosome in three conformational states.,Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y Cell Res. 2018 Mar;28(3):307-322. doi: 10.1038/cr.2018.14. Epub 2018 Jan 23. PMID:29360106<ref>PMID:29360106</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 5z58" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Helicase 3D structures|Helicase 3D structures]]
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
*[[Sm-like protein 3D structures|Sm-like protein 3D structures]]
== References ==
<references/>
__TOC__
</SX>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Lei J]]
[[Category: Li L]]
[[Category: Shi Y]]
[[Category: Yan C]]
[[Category: Zhan X]]
[[Category: Zhang X]]
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