5z5d: Difference between revisions

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New page: '''Unreleased structure''' The entry 5z5d is ON HOLD Authors: Description: Category: Unreleased Structures
 
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'''Unreleased structure'''


The entry 5z5d is ON HOLD
==Crystal structure of a thermostable glycoside hydrolase family 43 {beta}-1,4-xylosidase from Geobacillus thermoleovorans IT-08==
<StructureSection load='5z5d' size='340' side='right'caption='[[5z5d]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5z5d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_thermoleovorans Geobacillus thermoleovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z5D FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z5d OCA], [https://pdbe.org/5z5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z5d RCSB], [https://www.ebi.ac.uk/pdbsum/5z5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z5d ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q2I2N4_GEOTH Q2I2N4_GEOTH]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Complete degradation of the xylan backbone of hemicellulosic plant cell walls requires the synergistic action of endo-xylanases and beta-1,4-xylosidases. While endo-xylanases produce xylooligosaccharides from xylan, beta-1,4-xylosidases degrade the xylooligosaccharides into xylose monomers. The glycoside hydrolase family 43 beta-1,4-xylosidase from Geobacillus thermoleovorans IT-08 is a promising, heat stable catalyst for the saccharification of hemicellulosic material into simple fermentable sugars, but it is competitively inhibited by its products arabinose and xylose. As a first step to help overcome this problem, we elucidated crystal structures of the enzyme in the unliganded form and with bound products, at 1.7-2.0 A resolution. The structures are very similar to those of other enzymes belonging to glycoside hydrolase family 43. Unexpectedly, the monosaccharides are bound in very different ways. Arabinose preferentially binds in subsite -1, while xylose exclusively interacts with subsite +1. These structures and sugar binding preferences suggest ways for improving the catalytic performance of the enzyme by rational mutational design.


Authors:  
Structural basis of product inhibition by arabinose and xylose of the thermostable GH43 beta-1,4-xylosidase from Geobacillus thermoleovorans IT-08.,Rohman A, van Oosterwijk N, Puspaningsih NNT, Dijkstra BW PLoS One. 2018 Apr 26;13(4):e0196358. doi: 10.1371/journal.pone.0196358., eCollection 2018. PMID:29698436<ref>PMID:29698436</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 5z5d" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Xylosidase 3D structures|Xylosidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Geobacillus thermoleovorans]]
[[Category: Large Structures]]
[[Category: Dijkstra BW]]
[[Category: Puspaningsih NNT]]
[[Category: Rohman A]]
[[Category: Van Oosterwijk N]]
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