6c68: Difference between revisions
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New page: '''Unreleased structure''' The entry 6c68 is ON HOLD Authors: Lu, J., Van Laethem, F., Saba, I., Chu, J., Bhattacharya, A., Love, N.C., Tikhonova, A., Radaev, S., Sun, X., Ko, A., Arnon... |
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==MHC-independent t cell receptor A11== | |||
<StructureSection load='6c68' size='340' side='right'caption='[[6c68]], [[Resolution|resolution]] 2.59Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6c68]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6C68 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.59Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6c68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c68 OCA], [https://pdbe.org/6c68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6c68 RCSB], [https://www.ebi.ac.uk/pdbsum/6c68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6c68 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
During normal T cell development in the thymus, alphabeta TCRs signal immature thymocytes to differentiate into mature T cells by binding to peptide-MHC ligands together with CD4/CD8 coreceptors. Conversely, in MHC and CD4/CD8 coreceptor-deficient mice, the thymus generates mature T cells expressing MHC-independent TCRs that recognize native conformational epitopes rather than linear antigenic-peptides presented by MHC. To date, no structural information of MHC-independent TCRs is available, and their structural recognition of non-MHC ligand remains unknown. To our knowledge in this study, we determined the first structures of two murine MHC-independent TCRs (A11 and B12A) that bind with high nanomolar affinities to mouse adhesion receptor CD155. Solution binding demonstrated the Valphabeta-domain is responsible for MHC-independent B12A recognition of its ligand. Analysis of A11 and B12A sequences against various MHC-restricted and -independent TCR sequence repertoires showed that individual V-genes of A11 and B12A did not exhibit preference against MHC-restriction. Likewise, CDR3 alone did not discriminate against MHC binding, suggesting VDJ recombination together with Valpha/Vbeta pairing determine their MHC-independent specificity for CD155. The structures of A11 and B12A TCR are nearly identical to those of MHC-restricted TCR, including the conformations of CDR1 and 2. Mutational analysis, together with negative-staining electron microscopy images, showed that the CDR regions of A11 and B12A recognized epitopes on D1 domain of CD155, a region also involved in CD155 binding to poliovirus and Tactile in human. Taken together, MHC-independent TCRs adopt canonical TCR structures to recognize native Ags, highlighting the importance of thymic selection in determining TCR ligand specificity. | |||
Structure of MHC-Independent TCRs and Their Recognition of Native Antigen CD155.,Lu J, Van Laethem F, Saba I, Chu J, Tikhonova AN, Bhattacharya A, Singer A, Sun PD J Immunol. 2020 Jun 15;204(12):3351-3359. doi: 10.4049/jimmunol.1901084. Epub, 2020 Apr 22. PMID:32321756<ref>PMID:32321756</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6c68" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: | *[[T-cell receptor 3D structures|T-cell receptor 3D structures]] | ||
[[Category: Ko | == References == | ||
[[Category: | <references/> | ||
[[Category: Lu | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Mus musculus]] | ||
[[Category: | [[Category: Arnon T]] | ||
[[Category: | [[Category: Bhattacharya A]] | ||
[[Category: | [[Category: Chu J]] | ||
[[Category: | [[Category: Friedman N]] | ||
[[Category: | [[Category: Ko A]] | ||
[[Category: | [[Category: Love NC]] | ||
[[Category: Lu J]] | |||
[[Category: Radaev S]] | |||
[[Category: Saba I]] | |||
[[Category: Shifrut E]] | |||
[[Category: Singer A]] | |||
[[Category: Sun PD]] | |||
[[Category: Sun X]] | |||
[[Category: Tikhonova A]] | |||
[[Category: Van Laethem F]] | |||
[[Category: Weng N]] | |||
Latest revision as of 09:46, 23 October 2024
MHC-independent t cell receptor A11
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