1z26: Difference between revisions

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==Structure of Pyrococcus furiosus Argonaute with bound tungstate==
==Structure of Pyrococcus furiosus Argonaute with bound tungstate==
<StructureSection load='1z26' size='340' side='right' caption='[[1z26]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='1z26' size='340' side='right'caption='[[1z26]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1z26]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z26 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z26 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1z26]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z26 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z26 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1u04|1u04]], [[1z25|1z25]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z26 OCA], [http://pdbe.org/1z26 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z26 RCSB], [http://www.ebi.ac.uk/pdbsum/1z26 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1z26 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z26 OCA], [https://pdbe.org/1z26 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z26 RCSB], [https://www.ebi.ac.uk/pdbsum/1z26 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z26 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AGO_PYRFU AGO_PYRFU] A DNA-guided ssDNA endonuclease that may play a role in defense against invading mobile genetic elements. Uses short 5'-phospho-ssDNA sequences as guides (gDNA) to bind complementary target strands, resulting in cleavage of the target DNA (tDNA). Endonucleolytically cleaves DNA in short dsDNA (the gDNA indicates where to cleave on the tDNA). Efficient guide-dependent target DNA cleavage requires a minimal gDNA length of 15 nucleotides (nt) and works up to at least 31 nt. Overexpression decreases plasmid transformation efficiency. Has no appreciable activity with gRNA or on target RNA. Also has guide-independent activity on plasmid DNA called 'chopping' (PubMed:25925567). The cleavage site is 10 nucleotides (nt) downstream of the target residue base-paired with the 5'-end of the gDNA, cleavage is insensitive to adenine methylation. DNA cleavage produces 5'-phosphomonoesters (as it can be ligated by T4 DNA ligase) (PubMed:28165224).<ref>PMID:25925567</ref> <ref>PMID:28165224</ref>
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z2/1z26_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z2/1z26_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
   </jmolCheckbox>
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</div>
</div>
<div class="pdbe-citations 1z26" style="background-color:#fffaf0;"></div>
<div class="pdbe-citations 1z26" style="background-color:#fffaf0;"></div>
==See Also==
*[[Argonaute 3D structures|Argonaute 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc 43587]]
[[Category: Large Structures]]
[[Category: Aragon, J P]]
[[Category: Pyrococcus furiosus]]
[[Category: Hannon, G J]]
[[Category: Aragon JP]]
[[Category: Joshua-Tor, L]]
[[Category: Hannon GJ]]
[[Category: Liu, J]]
[[Category: Joshua-Tor L]]
[[Category: Rivas, F V]]
[[Category: Liu J]]
[[Category: Song, J J]]
[[Category: Rivas FV]]
[[Category: Tolia, N H]]
[[Category: Song JJ]]
[[Category: Argonaute]]
[[Category: Tolia NH]]
[[Category: Gene regulation]]
[[Category: Rnai]]
[[Category: Tungstate]]
Retrieved from "https://proteopedia.org/1z26"