6c9u: Difference between revisions

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New page: '''Unreleased structure''' The entry 6c9u is ON HOLD Authors: Deis, L.N., Li, X., Mathews, I.I., Khosla, C. Description: Crystal structure of [KS3][AT3] didomain from module 3 of 6-deo...
 
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'''Unreleased structure'''


The entry 6c9u is ON HOLD
==Crystal structure of [KS3][AT3] didomain from module 3 of 6-deoxyerthronolide B synthase in complex with antibody fragment (Fab)==
<StructureSection load='6c9u' size='340' side='right'caption='[[6c9u]], [[Resolution|resolution]] 2.09&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6c9u]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C9U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6C9U FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.09&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6c9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c9u OCA], [https://pdbe.org/6c9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6c9u RCSB], [https://www.ebi.ac.uk/pdbsum/6c9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6c9u ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ERYA2_SACER ERYA2_SACER]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Catalytic modules of assembly-line polyketide synthases (PKSs) have previously been observed in two very different conformations-an "extended" architecture and an "arch-shaped" architecture-although the catalytic relevance of neither has been directly established. By the use of a fully human naive antigen-binding fragment (Fab) library, a high-affinity antibody was identified that bound to the extended conformation of a PKS module, as verified by X-ray crystallography and tandem size-exclusion chromatography-small-angle X-ray scattering (SEC-SAXS). Kinetic analysis proved that this antibody-stabilized module conformation was fully competent for catalysis of intermodular polyketide chain translocation as well as intramodular polyketide chain elongation and functional group modification of a growing polyketide chain. Thus, the extended conformation of a PKS module is fully competent for all of its essential catalytic functions.


Authors: Deis, L.N., Li, X., Mathews, I.I., Khosla, C.
Structure-Function Analysis of the Extended Conformation of a Polyketide Synthase Module.,Li X, Sevillano N, La Greca F, Deis L, Liu YC, Deller MC, Mathews II, Matsui T, Cane DE, Craik CS, Khosla C J Am Chem Soc. 2018 May 18. doi: 10.1021/jacs.8b02100. PMID:29762030<ref>PMID:29762030</ref>


Description: Crystal structure of [KS3][AT3] didomain from module 3 of 6-deoxyerthronolide B synthase in complex with antibody fragment (Fab)
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Mathews, I.I]]
<div class="pdbe-citations 6c9u" style="background-color:#fffaf0;"></div>
[[Category: Deis, L.N]]
 
[[Category: Khosla, C]]
==See Also==
[[Category: Li, X]]
*[[Antibody 3D structures|Antibody 3D structures]]
*[[6-deoxyerythronolide B synthase 3D structures|6-deoxyerythronolide B synthase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Saccharopolyspora erythraea]]
[[Category: Deis LN]]
[[Category: Khosla C]]
[[Category: Li X]]
[[Category: Mathews II]]
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