2eh7: Difference between revisions

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[[Image:2eh7.jpg|left|200px]]


{{Structure
==Crystal structure of humanized KR127 FAB==
|PDB= 2eh7 |SIZE=350|CAPTION= <scene name='initialview01'>2eh7</scene>, resolution 2.50&Aring;
<StructureSection load='2eh7' size='340' side='right'caption='[[2eh7]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[2eh7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EH7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EH7 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
|GENE= HUMANIZED KR127 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eh7 OCA], [https://pdbe.org/2eh7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eh7 RCSB], [https://www.ebi.ac.uk/pdbsum/2eh7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eh7 ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=[[2eh8|2EH8]]
== Evolutionary Conservation ==
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eh7 OCA], [http://www.ebi.ac.uk/pdbsum/2eh7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2eh7 RCSB]</span>
[[Image:Consurf_key_small.gif|200px|right]]
}}
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eh/2eh7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eh7 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The humanized monoclonal antibody HzKR127 recognizes the preS1 domain of the human hepatitis B virus surface proteins with a broadly neutralizing activity in vivo. We present the crystal structures of HzKR127 Fab and its complex with a major epitope peptide. In the complex structure, the bound peptide forms a type IV beta-turn followed by 3(10) helical turn, the looped-out conformation of which provides a structural basis for broad neutralization. Upon peptide binding, the antibody undergoes a dramatic complementarity determining region H3 lid opening. To understand the structural implication of the virus neutralization, we carried out comprehensive alanine-scanning mutagenesis of all complementarity determining region residues in HzKR127 Fab. The functional mapping of the antigen-combining site demonstrates the specific roles of major binding determinants in antigen binding, contributing to the rational design for maximal humanization and affinity maturation of the antibody.


'''Crystal structure of humanized KR127 FAB'''
Broadly neutralizing anti-hepatitis B virus antibody reveals a complementarity determining region H3 lid-opening mechanism.,Chi SW, Maeng CY, Kim SJ, Oh MS, Ryu CJ, Kim SJ, Han KH, Hong HJ, Ryu SE Proc Natl Acad Sci U S A. 2007 May 29;104(22):9230-5. Epub 2007 May 17. PMID:17517649<ref>PMID:17517649</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2eh7" style="background-color:#fffaf0;"></div>


==Overview==
==See Also==
The humanized monoclonal antibody HzKR127 recognizes the preS1 domain of the human hepatitis B virus surface proteins with a broadly neutralizing activity in vivo. We present the crystal structures of HzKR127 Fab and its complex with a major epitope peptide. In the complex structure, the bound peptide forms a type IV beta-turn followed by 3(10) helical turn, the looped-out conformation of which provides a structural basis for broad neutralization. Upon peptide binding, the antibody undergoes a dramatic complementarity determining region H3 lid opening. To understand the structural implication of the virus neutralization, we carried out comprehensive alanine-scanning mutagenesis of all complementarity determining region residues in HzKR127 Fab. The functional mapping of the antigen-combining site demonstrates the specific roles of major binding determinants in antigen binding, contributing to the rational design for maximal humanization and affinity maturation of the antibody.
*[[Antibody 3D structures|Antibody 3D structures]]
 
*[[Sandbox 20009|Sandbox 20009]]
==About this Structure==
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
2EH7 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EH7 OCA].
== References ==
 
<references/>
==Reference==
__TOC__
Broadly neutralizing anti-hepatitis B virus antibody reveals a complementarity determining region H3 lid-opening mechanism., Chi SW, Maeng CY, Kim SJ, Oh MS, Ryu CJ, Kim SJ, Han KH, Hong HJ, Ryu SE, Proc Natl Acad Sci U S A. 2007 May 29;104(22):9230-5. Epub 2007 May 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17517649 17517649]
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Chi S-W]]
[[Category: Chi, S W.]]
[[Category: Hong HJ]]
[[Category: Hong, H J.]]
[[Category: Kim S-J]]
[[Category: Kim, S J.]]
[[Category: Maeng C-Y]]
[[Category: Maeng, C Y.]]
[[Category: Ryu S-E]]
[[Category: Ryu, S E.]]
[[Category: crystal structure]]
[[Category: hepatitis b virus]]
[[Category: humanized antibody]]
[[Category: immune system]]
[[Category: monoclonal antibody]]
[[Category: neutralization]]
[[Category: pres1]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:49:03 2008''
Retrieved from "https://proteopedia.org/2eh7"