2ek8: Difference between revisions

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[[Image:2ek8.jpg|left|200px]]


{{Structure
==Aminopeptidase from Aneurinibacillus sp. strain AM-1==
|PDB= 2ek8 |SIZE=350|CAPTION= <scene name='initialview01'>2ek8</scene>, resolution 1.80&Aring;
<StructureSection load='2ek8' size='340' side='right'caption='[[2ek8]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
|SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Residue+A+1001'>AC1</scene>, <scene name='pdbsite=AC2:Zn+Binding+Site+For+Residue+A+1002'>AC2</scene>, <scene name='pdbsite=AC3:Zn+Binding+Site+For+Residue+A+1003'>AC3</scene>, <scene name='pdbsite=AC4:Zn+Binding+Site+For+Residue+A+1004'>AC4</scene>, <scene name='pdbsite=AC5:Zn+Binding+Site+For+Residue+A+1005'>AC5</scene>, <scene name='pdbsite=AC6:Zn+Binding+Site+For+Residue+A+1006'>AC6</scene> and <scene name='pdbsite=AC7:Ipa+Binding+Site+For+Residue+A+2001'>AC7</scene>
== Structural highlights ==
|LIGAND= <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
<table><tr><td colspan='2'>[[2ek8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aneurinibacillus_sp._AM-1 Aneurinibacillus sp. AM-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EK8 FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
|GENE=
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam04389 Peptidase_M28], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd02133 PA_C5a_like]</span>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ek8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ek8 OCA], [https://pdbe.org/2ek8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ek8 RCSB], [https://www.ebi.ac.uk/pdbsum/2ek8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ek8 ProSAT]</span></td></tr>
|RELATEDENTRY=[[2ek9|2EK9]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ek8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ek8 OCA], [http://www.ebi.ac.uk/pdbsum/2ek8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ek8 RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/A2V759_9BACL A2V759_9BACL]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ek/2ek8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ek8 ConSurf].
<div style="clear:both"></div>


'''Aminopeptidase from Aneurinibacillus sp. strain AM-1'''
==See Also==
 
*[[Aminopeptidase 3D structures|Aminopeptidase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
To elucidate the structure and molecular mechanism of a characteristic proline-specific aminopeptidase produced by the thermophile Aneurinibacillus sp. strain AM-1, its gene was cloned and the recombinant protein was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.8 A resolution from the recombinant aminopeptidase crystal. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 93.62, b = 68.20, c = 76.84 A. A complete data set was also obtained from crystals of SeMet-substituted aminopeptidase. Data in the resolution range 20-2.1 A from the MAD data set from the SeMet-substituted crystal were used for phase determination.
[[Category: Aneurinibacillus sp. AM-1]]
 
[[Category: Large Structures]]
==About this Structure==
[[Category: Akioka M]]
2EK8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EK8 OCA].
[[Category: Nakano H]]
 
[[Category: Watanabe K]]
==Reference==
Overexpression, purification, crystallization and preliminary X-ray crystallographic studies of a proline-specific aminopeptidase from Aneurinibacillus sp. strain AM-1., Akioka M, Nakano H, Horikiri A, Tsujimoto Y, Matsui H, Shimizu T, Nakatsu T, Kato H, Watanabe K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Dec 1;62(Pt, 12):1266-8. Epub 2006 Nov 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17142913 17142913]
[[Category: Bacteria]]
[[Category: Single protein]]
[[Category: Akioka, M.]]
[[Category: Nakano, H.]]
[[Category: Watanabe, K.]]
[[Category: hydrolase]]
[[Category: metalloproteinase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:50:27 2008''
Retrieved from "https://proteopedia.org/2ek8"