Art:Ebola GP fusion loop: Difference between revisions

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[[Image:PDBe.png|150 px|right]]

[[Image:January2017.png|400 px|right]]

'''Behind the Artwork and the Protein Structure'''

The artist who created this image is Anna Valchanova, a student at Stephen Perse School in Cambridge, UK. She has presented the protein inside a drop of blood to reflect the blood transmission of the disease, and depicted the protein structure in bleach to reflect the decline of health once infected with Ebola virus. Anna has based her work on the GP ‘fusion loop’ structure, solved by NMR spectroscopy in the Tamm lab, in the University of Virginia, School of Medicine. ~~Further information about this structure can be found~~ in PDB ~~entry~~ 2m5f

The artist who created this image is Anna Valchanova, a student at Stephen Perse School in Cambridge, UK. She has presented the protein inside a drop of blood to reflect the blood transmission of the disease, and depicted the protein structure in bleach to reflect the decline of health once infected with Ebola virus. Anna has based her work on the GP ‘fusion loop’ structure, solved by NMR spectroscopy in the Tamm lab, in the University of Virginia, School of Medicine.

View in 3D <jmol>

<urlcontents>/cgi-bin/getlateststructure?2m5f.gz;load /wiki/extensions/Proteopedia/spt/initialview01.spt</urlcontents>

<text>Explore</text>

</jmolAppletButton>

</jmol> or go to PDB structure [[2m5f]] <ref>pmid 24696482</ref>

'''Ebola Virus'''

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GP is shaped like a three pronged bowl and is anchored in the viral membrane by three helices, and is the protein responsible for getting the virus into the cytoplasm of a host cell. Mature GP is composed of two chains, termed GP1 and GP2, linked by a disulphide bond, between two cysteine amino acids. GP1 is responsible for binding to host cell receptors during infection and forms most of the bowl while GP2 sits at the base of the bowl, tying the trimer together and mediating the fusion of the viral and host membranes. A crucial part of Ebola GP2 is the fusion loop. It lies flat against the surface of the GP trimer until a drop in pH trigers a conformational change. This change enables the tip of the loop to insert into host cell membranes and triggers the membrane fusion process.

Human antibodies, found in Ebola survivors (PDB entry 3csy), bind to the fusion loop These antibodies may act by preventing the change in shape of the loop and thus blocking the virus entering the human cells. The fusion loop in GP is also the site of action of drugs such as toremifene (PDB entry 5jq7) and ibuprofen (PDB entry 5jqb) which are thought to destabilise the fusion process and could lead to Ebola therapueutics.

Human antibodies, found in Ebola survivors (PDB entry [[3csy]]), bind to the fusion loop These antibodies may act by preventing the change in shape of the loop and thus blocking the virus entering the human cells. The fusion loop in GP is also the site of action of drugs such as toremifene (PDB entry [[5jq7]]) and ibuprofen (PDB entry [[5jqb]]) which are thought to destabilise the fusion process and could lead to Ebola therapueutics.

Once the tip of the fusion loop has inserted into the host membrane, GP2, including the fusion loop, undergoes a huge change in conformation. The post-fusion conformation of GP2 (PDB entry [[1ebo]]) is vastly different to that before membrane fusion and shows that the fusion loop becomes helical and moves to the vicinity of the viral membrane, likely dragging the host membrane with it and effecting membrane fusion.

'''Explore the scientific publication'''

Once the tip of the fusion loop has inserted into the host membrane, GP2, including the fusion loop, undergoes a huge change in conformation. The post-fusion conformation of GP2 (PDB entry 1ebo) is vastly different to that before membrane fusion and shows that the fusion loop becomes helical and moves to the vicinity of the viral membrane, likely dragging the host membrane with it and effecting membrane fusion.

[[Category: PDB Art]]

Art:Ebola GP fusion loop: Difference between revisions

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@@ Line 1: / Line 1: @@
+[[Image:PDBe.png|150 px|right]]
 [[Image:January2017.png|400 px|right]]
 '''Behind the Artwork and the Protein Structure'''
-The artist who created this image is Anna Valchanova, a student at Stephen Perse School in Cambridge, UK. She has presented the protein inside a drop of blood to reflect the blood transmission of the disease, and depicted the protein structure in bleach to reflect the decline of health once infected with Ebola virus. Anna has based her work on the GP ‘fusion loop’ structure, solved by NMR spectroscopy in the Tamm lab, in the University of Virginia, School of Medicine. Further information about this structure can be found in PDB entry 2m5f
+The artist who created this image is Anna Valchanova, a student at Stephen Perse School in Cambridge, UK. She has presented the protein inside a drop of blood to reflect the blood transmission of the disease, and depicted the protein structure in bleach to reflect the decline of health once infected with Ebola virus. Anna has based her work on the GP ‘fusion loop’ structure, solved by NMR spectroscopy in the Tamm lab, in the University of Virginia, School of Medicine.
+View in 3D <jmol>
+  <jmolAppletButton>
+    <urlcontents>/cgi-bin/getlateststructure?2m5f.gz;load /wiki/extensions/Proteopedia/spt/initialview01.spt</urlcontents>
+    <text>Explore</text>
+  </jmolAppletButton>
+</jmol>  or go to PDB structure [[2m5f]] <ref>pmid 24696482</ref>
 '''Ebola Virus'''
@@ Line 13: / Line 22: @@
 GP is shaped like a three pronged bowl and is anchored in the viral membrane by three helices, and is the protein responsible for getting the virus into the cytoplasm of a host cell. Mature GP is composed of two chains, termed GP1 and GP2, linked by a disulphide bond, between two cysteine amino acids. GP1 is responsible for binding to host cell receptors during infection and forms most of the bowl while GP2 sits at the base of the bowl, tying the trimer together and mediating the fusion of the viral and host membranes. A crucial part of Ebola GP2 is the fusion loop. It lies flat against the surface of the GP trimer until a drop in pH trigers a conformational change. This change enables the tip of the loop to insert into host cell membranes and triggers the membrane fusion process.
-Human antibodies, found in Ebola survivors (PDB entry 3csy), bind to the fusion loop These antibodies may act by preventing the change in shape of the loop and thus blocking the virus entering the human cells. The fusion loop in GP is also the site of action of drugs such as toremifene (PDB entry 5jq7) and ibuprofen (PDB entry 5jqb) which are thought to destabilise the fusion process and could lead to Ebola therapueutics.
+Human antibodies, found in Ebola survivors (PDB entry [[3csy]]), bind to the fusion loop These antibodies may act by preventing the change in shape of the loop and thus blocking the virus entering the human cells. The fusion loop in GP is also the site of action of drugs such as toremifene (PDB entry [[5jq7]]) and ibuprofen (PDB entry [[5jqb]]) which are thought to destabilise the fusion process and could lead to Ebola therapueutics.
+Once the tip of the fusion loop has inserted into the host membrane, GP2, including the fusion loop, undergoes a huge change in conformation. The post-fusion conformation of GP2 (PDB entry [[1ebo]]) is vastly different to that before membrane fusion and shows that the fusion loop becomes helical and moves to the vicinity of the viral membrane, likely dragging the host membrane with it and effecting membrane fusion.
+'''Explore the scientific publication'''
+<references/>
-Once the tip of the fusion loop has inserted into the host membrane, GP2, including the fusion loop, undergoes a huge change in conformation. The post-fusion conformation of GP2 (PDB entry 1ebo) is vastly different to that before membrane fusion and shows that the fusion loop becomes helical and moves to the vicinity of the viral membrane, likely dragging the host membrane with it and effecting membrane fusion.
 [[Category: PDB Art]]