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[[Image:2er8.gif|left|200px]]


{{Structure
==Crystal Structure of Leu3 DNA-binding domain complexed with a 12mer DNA duplex==
|PDB= 2er8 |SIZE=350|CAPTION= <scene name='initialview01'>2er8</scene>, resolution 2.85&Aring;
<StructureSection load='2er8' size='340' side='right'caption='[[2er8]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
<table><tr><td colspan='2'>[[2er8]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ER8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ER8 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
|GENE= LEU3 (32-103) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2er8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2er8 OCA], [https://pdbe.org/2er8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2er8 RCSB], [https://www.ebi.ac.uk/pdbsum/2er8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2er8 ProSAT]</span></td></tr>
|RELATEDENTRY=[[2ere|2ERE]], [[2erg|2ERG]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2er8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2er8 OCA], [http://www.ebi.ac.uk/pdbsum/2er8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2er8 RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/LEUR_YEAST LEUR_YEAST] Factor for control of RNA levels of a group of leucine-specific genes.
 
== Evolutionary Conservation ==
'''Crystal Structure of Leu3 DNA-binding domain complexed with a 12mer DNA duplex'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/er/2er8_consurf.spt"</scriptWhenChecked>
Gal4 is the prototypical Zn2Cys6 binuclear cluster transcriptional regulator that binds as a homodimer to DNA containing inverted CGG half-sites. Leu3, a member of this protein family, binds to everted (opposite polarity to inverted) CGG half-sites, and an H50C mutation within the Leu3 Zn2Cys6 binuclear motif abolishes its transcriptional repression function without impairing DNA binding. We report the X-ray crystal structures of DNA complexes with Leu3 and Leu3(H50C) and solution DNA binding studies of selected Leu3 mutant proteins. These studies reveal the molecular details of everted CGG half-site recognition, and suggest a role for the H50C mutation in transcriptional repression. Comparison with the Gal4-DNA complex shows an unexpected conservation in the DNA recognition mode of inverted and everted CGG half-sites, and points to a critical function of a linker region between the Zn2Cys6 binuclear cluster and dimerization regions in DNA binding specificity. Broader implications of these findings are discussed.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
2ER8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ER8 OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2er8 ConSurf].
 
<div style="clear:both"></div>
==Reference==
__TOC__
Structure of a Leu3-DNA complex: recognition of everted CGG half-sites by a Zn2Cys6 binuclear cluster protein., Fitzgerald MX, Rojas JR, Kim JM, Kohlhaw GB, Marmorstein R, Structure. 2006 Apr;14(4):725-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16615914 16615914]
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Fitzgerald MX]]
[[Category: Fitzgerald, M X.]]
[[Category: Marmorstein R]]
[[Category: Marmorstein, R.]]
[[Category: zn(2)cys(6) binuclear cluster motif]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:52:44 2008''

Latest revision as of 09:19, 14 February 2024

Crystal Structure of Leu3 DNA-binding domain complexed with a 12mer DNA duplex

2er8, resolution 2.85Å

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