5zcn: Difference between revisions
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==Solution NMR structure of a new lasso peptide brevunsin== | |||
<StructureSection load='5zcn' size='340' side='right'caption='[[5zcn]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5zcn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZCN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZCN FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 15 models</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zcn OCA], [https://pdbe.org/5zcn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zcn RCSB], [https://www.ebi.ac.uk/pdbsum/5zcn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zcn ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A shuttle vector pHSG396Sp was constructed to perform gene expression using Sphingomonas subterranea as a host. A new lasso peptide biosynthetic gene cluster, derived from Brevundimonas diminuta, was amplified by PCR and integrated to afford a expression vector pHSG396Sp-12697L. The new lasso peptide brevunsin was successfully produced by S. subterranea, harboring the expression vector, with a high production yield (10.2 mg from 1 L culture). The chemical structure of brevunsin was established by NMR and MS/MS experiments. Based on the information obtained from the NOE experiment, the three-dimensional structure of brevunsin was determined, which indicated that brevunsin possessed a typical lasso structure. This expression vector system provides a new heterologous production method for unexplored lasso peptides that are encoded by bacterial genomes. | |||
Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea.,Kodani S, Hemmi H, Miyake Y, Kaweewan I, Nakagawa H J Ind Microbiol Biotechnol. 2018 Nov;45(11):983-992. doi:, 10.1007/s10295-018-2077-6. Epub 2018 Sep 6. PMID:30191430<ref>PMID:30191430</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5zcn" style="background-color:#fffaf0;"></div> | ||
[[Category: Hemmi | == References == | ||
[[Category: Kaweewan | <references/> | ||
[[Category: Miyake | __TOC__ | ||
[[Category: Nakagawa | </StructureSection> | ||
[[Category: Brevundimonas diminuta]] | |||
[[Category: Large Structures]] | |||
[[Category: Hemmi H]] | |||
[[Category: Kaweewan I]] | |||
[[Category: Kodani S]] | |||
[[Category: Miyake Y]] | |||
[[Category: Nakagawa H]] | |||
Latest revision as of 08:55, 9 October 2024
Solution NMR structure of a new lasso peptide brevunsin
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