6ftb

Staphylococcus aureus monofunctional glycosyltransferase in complex with moenomycin

spinqualitylabelsall models 6ftb, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ftb is a 1 chain structure with sequence from Staphylococcus aureus mw2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 1QW, EDO, M0E, PO4 Related: 3hzs, 2c6w, 2zc5, 2olu, 3dwk, 2olv Gene: mgt, MW1814 (Staphylococcus aureus MW2) Activity: Peptidoglycan glycosyltransferase, with EC number 2.4.1.129 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [MGT_STAAW] Involved in the biosynthesis of cell wall peptidoglycan. Responsible for the elongation of the glycan strands using lipid-linked disaccharide-pentapeptide as the substrate (By similarity). Publication Abstract from PubMed Bacterial peptidoglycan glycosyltransferases (PGT) catalyse the essential polymerization of lipid II into linear glycan chains required for peptidoglycan biosynthesis. The PGT domain is composed of a large head subdomain and a smaller jaw subdomain and can be potently inhibited by the antibiotic moenomycin A (MoeA). We present an X-ray structure of the MoeA-bound Staphylococcus aureus monofunctional PGT enzyme, revealing electron density for a second MoeA bound to the jaw subdomain as well as the PGT donor site. Isothermal titration calorimetry confirms two drug-binding sites with markedly different affinities and positive cooperativity. Hydrophobic cluster analysis suggests that the membrane-interacting surface of the jaw subdomain has structural and physicochemical properties similar to amphipathic cationic alpha -helical antimicrobial peptides for lipid II recognition and binding. Furthermore, molecular dynamics simulations of the drug-free and -bound forms of the enzyme demonstrate the importance of the jaw subdomain movement for lipid II selection and polymerization process and provide molecular-level insights into the mechanism of peptidoglycan biosynthesis by PGTs. The role of the jaw subdomain of peptidoglycan glycosyltransferases for lipid II polymerization.,Punekar AS, Samsudin F, Lloyd AJ, Dowson CG, Scott DJ, Khalid S, Roper DI Cell Surf. 2018 Jun;2:54-66. doi: 10.1016/j.tcsw.2018.06.002. PMID:30046666 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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