5n4a: Difference between revisions
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==Crystal structure of Chlamydomonas IFT80== | ==Crystal structure of Chlamydomonas IFT80== | ||
<StructureSection load='5n4a' size='340' side='right' caption='[[5n4a]], [[Resolution|resolution]] 1.79Å' scene=''> | <StructureSection load='5n4a' size='340' side='right'caption='[[5n4a]], [[Resolution|resolution]] 1.79Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5n4a]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N4A OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[5n4a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N4A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N4A FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.79Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n4a OCA], [https://pdbe.org/5n4a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n4a RCSB], [https://www.ebi.ac.uk/pdbsum/5n4a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n4a ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/A5Z0S9_CHLRE A5Z0S9_CHLRE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Oligomeric assemblies of intraflagellar transport (IFT) particles build cilia through sequential recruitment and transport of ciliary cargo proteins within cilia. Here we present the 1.8 A resolution crystal structure of the Chlamydomonas IFT-B protein IFT80, which reveals the architecture of two N-terminal beta-propellers followed by an alpha-helical extension. The N-terminal beta-propeller tethers IFT80 to the IFT-B complex via IFT38 whereas the second beta-propeller and the C-terminal alpha-helical extension result in IFT80 homo-dimerization. Using CRISPR/Cas to create biallelic Ift80 frameshift mutations in IMCD3 mouse cells, we demonstrate that IFT80 is absolutely required for ciliogenesis. Structural mapping and rescue experiments reveal that human disease-causing missense mutations do not cluster within IFT80 and form functional IFT particles. Unlike missense mutant forms of IFT80, deletion of the C-terminal dimerization domain prevented rescue of ciliogenesis. Taken together our results may provide a first insight into higher order IFT complex formation likely required for IFT train formation. | |||
Crystal structure of intraflagellar transport protein 80 reveals a homo-dimer required for ciliogenesis.,Taschner M, Lorentzen A, Mourao A, Collins T, Freke GM, Moulding D, Basquin J, Jenkins D, Lorentzen E Elife. 2018 Apr 16;7. pii: 33067. doi: 10.7554/eLife.33067. PMID:29658880<ref>PMID:29658880</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5n4a" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Chlamydomonas reinhardtii]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Mourao A]] | ||
[[Category: | [[Category: Taschner M]] | ||
Latest revision as of 18:01, 8 November 2023
Crystal structure of Chlamydomonas IFT80
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