6chv: Difference between revisions
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The | ==Proteus vulgaris HigA antitoxin bound to DNA== | ||
<StructureSection load='6chv' size='340' side='right'caption='[[6chv]], [[Resolution|resolution]] 2.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6chv]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CHV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CHV FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6chv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6chv OCA], [https://pdbe.org/6chv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6chv RCSB], [https://www.ebi.ac.uk/pdbsum/6chv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6chv ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/HIGA_PROVU HIGA_PROVU] Antitoxin component of a type II toxin-antitoxin (TA) system that counteracts the effect of the HigB toxin (PubMed:19423702, PubMed:8645296, PubMed:24257752). Binds to its own promoter and regulates transcription of the higB/higA operon (PubMed:24257752).<ref>PMID:19423702</ref> <ref>PMID:24257752</ref> <ref>PMID:8645296</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacterial toxin-antitoxin systems are important factors implicated in growth inhibition and plasmid maintenance. Type II toxin-antitoxin pairs are regulated at the transcriptional level by the antitoxin itself. Here, we examined how the HigA antitoxin regulates the expression of the Proteus vulgaris higBA toxin-antitoxin operon from the Rts1 plasmid. The HigBA complex adopts a unique architecture suggesting differences in its regulation as compared to classical type II toxin-antitoxin systems. We find that the C-terminus of the HigA antitoxin is required for dimerization and transcriptional repression. Further, the HigA structure reveals that the C terminus is ordered and does not transition between disorder-order states upon toxin binding. HigA residue Arg40 recognizes a TpG dinucleotide in higO2, an evolutionary conserved mode of recognition among prokaryotic and eukaryotic transcriptional factors. Comparison of the HigBA and HigA-higO2 structures reveals the distance between helix-turn-helix motifs of each HigA monomer increases by ~4 A in order to bind to higO2. Consistent with these data, HigBA binding to each operator is two-fold less tight than HigA alone. Together, these data show the HigB toxin does not act as a co-repressor suggesting potential novel regulation in this toxin-antitoxin system. This article is protected by copyright. All rights reserved. | |||
Structural basis of transcriptional regulation by the HigA antitoxin.,Schureck MA, Meisner J, Hoffer ED, Wang D, Onuoha N, Ei Cho S, Lollar P 3rd, Dunham CM Mol Microbiol. 2019 Feb 21. doi: 10.1111/mmi.14229. PMID:30793388<ref>PMID:30793388</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6chv" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Proteus vulgaris]] | |||
[[Category: Dunham CM]] | |||
[[Category: Hoffer ED]] | |||
[[Category: Onuoha N]] | |||
[[Category: Schureck MA]] | |||
Latest revision as of 15:05, 4 October 2023
Proteus vulgaris HigA antitoxin bound to DNA
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