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New page: '''Unreleased structure''' The entry 6ck5 is ON HOLD Authors: Description: Category: Unreleased Structures
 
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'''Unreleased structure'''


The entry 6ck5 is ON HOLD
==PRPP riboswitch from T. mathranii bound to PRPP==
<StructureSection load='6ck5' size='340' side='right'caption='[[6ck5]], [[Resolution|resolution]] 2.49&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ck5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoanaerobacter_mathranii Thermoanaerobacter mathranii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CK5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CK5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.49&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRP:ALPHA-PHOSPHORIBOSYLPYROPHOSPHORIC+ACID'>PRP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ck5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ck5 OCA], [https://pdbe.org/6ck5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ck5 RCSB], [https://www.ebi.ac.uk/pdbsum/6ck5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ck5 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Two classes of riboswitches related to the ykkC guanidine-I riboswitch bind phosphoribosyl pyrophosphate (PRPP) and guanosine tetraphosphate (ppGpp). Here we report the co-crystal structure of the PRPP aptamer and its ligand. We also report the structure of the G96A point mutant that prefers ppGpp over PRPP with a dramatic 40,000-fold switch in specificity. The ends of the aptamer form a helix that is not present in the guanidine aptamer and is involved in the expression platform. In the mutant, the base of ppGpp replaces G96 in three-dimensional space. This disrupts the S-turn, which is a primary structural feature of the ykkC RNA motif. These dramatic differences in ligand specificity are achieved with minimal mutations. ykkC aptamers are therefore a prime example of an RNA fold with a rugged fitness landscape. The ease with which the ykkC aptamer acquires new specificity represents a striking case of evolvability in RNA.


Authors:  
Structures of two aptamers with differing ligand specificity reveal ruggedness in the functional landscape of RNA.,Knappenberger AJ, Reiss CW, Strobel SA Elife. 2018 Jun 7;7. pii: 36381. doi: 10.7554/eLife.36381. PMID:29877798<ref>PMID:29877798</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 6ck5" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Riboswitch 3D structures|Riboswitch 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermoanaerobacter mathranii]]
[[Category: Knappenberger AJ]]
[[Category: Reiss CW]]
[[Category: Strobel SA]]
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