5v7m: Difference between revisions

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==PCNA mutant L126A/I128A Protein Defective in Gene Silencing==
==PCNA mutant L126A/I128A Protein Defective in Gene Silencing==
<StructureSection load='5v7m' size='340' side='right' caption='[[5v7m]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
<StructureSection load='5v7m' size='340' side='right'caption='[[5v7m]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5v7m]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5V7M FirstGlance]. <br>
<table><tr><td colspan='2'>[[5v7m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V7M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5V7M FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5v7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v7m OCA], [http://pdbe.org/5v7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5v7m RCSB], [http://www.ebi.ac.uk/pdbsum/5v7m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5v7m ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5v7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v7m OCA], [https://pdbe.org/5v7m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5v7m RCSB], [https://www.ebi.ac.uk/pdbsum/5v7m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5v7m ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/PCNA_YEAST PCNA_YEAST]] This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.<ref>PMID:11545742</ref> <ref>PMID:12226657</ref>
[https://www.uniprot.org/uniprot/PCNA_YEAST PCNA_YEAST] This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.<ref>PMID:11545742</ref> <ref>PMID:12226657</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Proliferating cell nuclear antigen (PCNA), a homotrimeric protein, is the eukaryotic sliding clamp that functions as a processivity factor for polymerases during DNA replication. Chromatin association factor 1 (CAF-1) is a heterotrimeric histone chaperone protein that is required for coupling chromatin assembly with DNA replication in eukaryotes. CAF-1 association with replicating DNA, and the targeting of newly synthesized histones to sites of DNA replication and repair requires its interaction with PCNA. Genetic studies have identified three mutant forms of PCNA in yeast that cause defects in gene silencing and exhibit altered association of CAF-1 to chromatin in vivo, as well as inhibit binding to CAF-1 in vitro. Three of these mutant forms of PCNA, encoded by the pol30-6, pol30-8, and the pol30-79 alleles, direct the synthesis of PCNA proteins with the amino acid substitutions D41A/D42A, R61A/D63A, and L126A/I128A, respectively. Interestingly, these double alanine substitutions are located far away from each other within the PCNA protein. To understand the structural basis of the interaction between PCNA and CAF-1 and how disruption of this interaction leads to reduced gene silencing, we determined the X-ray crystal structures of each of these mutant PCNA proteins. All three of the substitutions caused disruptions of a surface cavity on the front face of the PCNA ring, which is formed in part by three loops comprised of residues 21-24, 41-44, and 118-134. We suggest that this cavity is a novel binding pocket required for the interaction between PCNA and CAF-1, and that this region in PCNA also represents a potential binding site for other PCNA-binding proteins.


Crystal structures of PCNA mutant proteins defective in gene silencing suggest a novel interaction site on the front face of the PCNA ring.,Kondratick CM, Litman JM, Shaffer KV, Washington MT, Dieckman LM PLoS One. 2018 Mar 2;13(3):e0193333. doi: 10.1371/journal.pone.0193333., eCollection 2018. PMID:29499038<ref>PMID:29499038</ref>
==See Also==
 
*[[Proliferating cell nuclear antigen 3D structures|Proliferating cell nuclear antigen 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5v7m" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Dieckman, L M]]
[[Category: Large Structures]]
[[Category: Kondratick, C M]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Litman, J M]]
[[Category: Dieckman LM]]
[[Category: Washington, M T]]
[[Category: Kondratick CM]]
[[Category: Caf-1]]
[[Category: Litman JM]]
[[Category: Dna binding protein]]
[[Category: Washington MT]]

Latest revision as of 14:31, 6 March 2024

PCNA mutant L126A/I128A Protein Defective in Gene Silencing

5v7m, resolution 1.93Å

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