6g0l: Difference between revisions
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New page: '''Unreleased structure''' The entry 6g0l is ON HOLD until sometime in the future Authors: Sundaramoorthy, R., Owen-hughes, T., Norman, D.G., Hughes, A. Description: Structure of the c... |
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==Structure of two molecules of the chromatin remodelling enzyme Chd1 bound to a nucleosome== | |||
<SX load='6g0l' size='340' side='right' viewer='molstar' caption='[[6g0l]], [[Resolution|resolution]] 10.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6g0l]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C], [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G0L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6G0L FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 10Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6g0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g0l OCA], [https://pdbe.org/6g0l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6g0l RCSB], [https://www.ebi.ac.uk/pdbsum/6g0l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6g0l ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q92133_XENLA Q92133_XENLA] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
ATP-dependent chromatin remodelling proteins represent a diverse family of proteins that share ATPase domains that are adapted to regulate protein-DNA interactions. Here we present structures of the Saccharomyces cerevisiae Chd1 protein engaged with nucleosomes in the presence of the transition state mimic ADP-beryllium fluoride. The path of DNA strands through the ATPase domains indicates the presence of contacts conserved with single strand translocases and additional contacts with both strands that are unique to Snf2 related proteins. The structure provides connectivity between rearrangement of ATPase lobes to a closed, nucleotide bound state and the sensing of linker DNA. Two turns of linker DNA are prised off the surface of the histone octamer as a result of Chd1 binding, and both the histone H3 tail and ubiquitin conjugated to lysine 120 are re-orientated towards the unravelled DNA. This indicates how changes to nucleosome structure can alter the way in which histone epitopes are presented. | |||
Structure of the chromatin remodelling enzyme Chd1 bound to a ubiquitinylated nucleosome.,Sundaramoorthy R, Hughes AL, El-Mkami H, Norman DG, Ferreira H, Owen-Hughes T Elife. 2018 Aug 6;7. pii: 35720. doi: 10.7554/eLife.35720. PMID:30079888<ref>PMID:30079888</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6g0l" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: Hughes | ==See Also== | ||
[[Category: Norman | *[[Chromodomain-helicase-DNA-binding protein 3D structures|Chromodomain-helicase-DNA-binding protein 3D structures]] | ||
*[[Helicase 3D structures|Helicase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Large Structures]] | |||
[[Category: Saccharomyces cerevisiae S288C]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Xenopus laevis]] | |||
[[Category: Hughes A]] | |||
[[Category: Norman DG]] | |||
[[Category: Owen-hughes T]] | |||
[[Category: Sundaramoorthy R]] | |||
Latest revision as of 17:07, 25 September 2026
Structure of two molecules of the chromatin remodelling enzyme Chd1 bound to a nucleosome
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