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==Crystal structure of the receptor-binding domain of the FA hybrid Clostridium botulinum neurotoxin==
==Crystal structure of the receptor-binding domain of the FA hybrid Clostridium botulinum neurotoxin==
<StructureSection load='5mk8' size='340' side='right' caption='[[5mk8]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
<StructureSection load='5mk8' size='340' side='right'caption='[[5mk8]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5mk8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MK8 FirstGlance]. <br>
<table><tr><td colspan='2'>[[5mk8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MK8 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mk8 OCA], [http://pdbe.org/5mk8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mk8 RCSB], [http://www.ebi.ac.uk/pdbsum/5mk8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mk8 ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mk8 OCA], [https://pdbe.org/5mk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mk8 RCSB], [https://www.ebi.ac.uk/pdbsum/5mk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mk8 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9R540_CLOBO Q9R540_CLOBO]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The binding specificity of botulinum neurotoxins (BoNTs) is primarily a consequence of their ability to bind to multiple receptors at the same time. BoNTs consist of three distinct domains, a metalloprotease light chain (LC), a translocation domain (HN) and a receptor-binding domain (HC). Here we report the crystal structure of HC/FA, complementing an existing structure through the modelling of a previously unresolved loop which is important for receptor-binding. Our HC/FA structure also contains a previously unidentified disulphide bond, which we have also observed in one of two crystal forms of HC/A1. This may have implications for receptor-binding and future recombinant toxin production.
High resolution crystal structures of the receptor-binding domain of Clostridium botulinum neurotoxin serotypes A and FA.,Davies JR, Hackett GS, Liu SM, Acharya KR PeerJ. 2018 Mar 21;6:e4552. doi: 10.7717/peerj.4552. eCollection 2018. PMID:29576992<ref>PMID:29576992</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5mk8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Acharya, K R]]
[[Category: Clostridium botulinum]]
[[Category: Davies, J R]]
[[Category: Large Structures]]
[[Category: Bacterial toxin toxin receptor binding domain jelly roll fold beta trefoil fold]]
[[Category: Acharya KR]]
[[Category: Toxin]]
[[Category: Davies JR]]
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