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[[Image:2g7o.gif|left|200px]]


{{Structure
==Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM==
|PDB= 2g7o |SIZE=350|CAPTION= <scene name='initialview01'>2g7o</scene>, resolution 1.400&Aring;
<StructureSection load='2g7o' size='340' side='right'caption='[[2g7o]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[2g7o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G7O FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
|GENE= traM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g7o OCA], [https://pdbe.org/2g7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g7o RCSB], [https://www.ebi.ac.uk/pdbsum/2g7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g7o ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=
== Function ==
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g7o OCA], [http://www.ebi.ac.uk/pdbsum/2g7o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2g7o RCSB]</span>
[https://www.uniprot.org/uniprot/TRAM1_ECOLI TRAM1_ECOLI] Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Cooperatively binds 3 regions in the F plasmid oriT locus; 2 are required for autoregulation while the other is required for plasmid transfer. Bends oriT DNA less than 50 degrees. Plasmid specificity is conferred by the TraD-TraM pair.<ref>PMID:1479887</ref> <ref>PMID:8736534</ref> <ref>PMID:11875064</ref> <ref>PMID:17238924</ref>
}}
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
'''Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM'''
Check<jmol>
 
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g7/2g7o_consurf.spt"</scriptWhenChecked>
==Overview==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
TraM is essential for F plasmid-mediated bacterial conjugation, where it binds to the plasmid DNA near the origin of transfer, and recognizes a component of the transmembrane DNA transfer complex, TraD. Here we report the 1.40 A crystal structure of the TraM core tetramer (TraM58-127). TraM58-127 is a compact eight-helical bundle, in which the N-terminal helices from each protomer interact to form a central, parallel four-stranded coiled-coil, whereas each C-terminal helix packs in an antiparallel arrangement around the outside of the structure. Four protonated glutamic acid residues (Glu88) are packed in a hydrogen-bonded arrangement within the central four-helix bundle. Mutational and biophysical analyses indicate that this protonated state is in equilibrium with a deprotonated tetrameric form characterized by a lower helical content at physiological pH and temperature. Comparison of TraM to its Glu88 mutants predicted to stabilize the helical structure suggests that the protonated state is the active form for binding TraD in conjugation.
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==About this Structure==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g7o ConSurf].
2G7O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G7O OCA].  
<div style="clear:both"></div>
 
== References ==
==Reference==
<references/>
Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM., Lu J, Edwards RA, Wong JJ, Manchak J, Scott PG, Frost LS, Glover JN, EMBO J. 2006 Jun 21;25(12):2930-9. Epub 2006 May 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16710295 16710295]
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Edwards, R A.]]
[[Category: Edwards RA]]
[[Category: Frost, L S.]]
[[Category: Frost LS]]
[[Category: Glover, J N.]]
[[Category: Glover JN]]
[[Category: Lu, J.]]
[[Category: Lu J]]
[[Category: Manchak, J.]]
[[Category: Manchak J]]
[[Category: Scott, P G.]]
[[Category: Scott PG]]
[[Category: Wong, J J.]]
[[Category: Wong JJ]]
[[Category: four helix bundle]]
[[Category: tetramer]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:12:58 2008''

Latest revision as of 09:26, 14 February 2024

Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM

2g7o, resolution 1.40Å

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