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[[Image:2geb.jpg|left|200px]]


{{Structure
==Crystal structure of the Thermoanaerobacter tengcongensis hypoxanthine-guanine phosphoribosyltransferase L160I mutant: insights into the inhibitor design==
|PDB= 2geb |SIZE=350|CAPTION= <scene name='initialview01'>2geb</scene>, resolution 1.70&Aring;
<StructureSection load='2geb' size='340' side='right'caption='[[2geb]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
<table><tr><td colspan='2'>[[2geb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GEB FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Hypoxanthine_phosphoribosyltransferase Hypoxanthine phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.8 2.4.2.8] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
|GENE= HGPRT, Hpt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=119072 Thermoanaerobacter tengcongensis])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2geb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2geb OCA], [https://pdbe.org/2geb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2geb RCSB], [https://www.ebi.ac.uk/pdbsum/2geb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2geb ProSAT]</span></td></tr>
|RELATEDENTRY=[[1r3u|1R3U]], [[1yfz|1YFZ]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2geb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2geb OCA], [http://www.ebi.ac.uk/pdbsum/2geb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2geb RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/Q8R7L0_CALS4 Q8R7L0_CALS4]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ge/2geb_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2geb ConSurf].
<div style="clear:both"></div>


'''Crystal structure of the Thermoanaerobacter tengcongensis hypoxanthine-guanine phosphoribosyltransferase L160I mutant: insights into the inhibitor design'''
==See Also==
 
*[[Phosphoribosyltransferase 3D structures|Phosphoribosyltransferase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) is a potential target for structure-based inhibitor design for the treatment of parasitic diseases. We created point mutants of Thermoanaerobacter tengcongensis HGPRT and tested their activities to identify side chains that were important for function. Mutating residues Leu160 and Lys133 substantially diminished the activity of HGPRT, confirming their importance in catalysis. All 11 HGPRT mutants were subject to crystallization screening. The crystal structure of one mutant, L160I, was determined at 1.7 A resolution. Surprisingly, the active site is occupied by a peptide from the N-terminus of a neighboring tetramer. These crystal contacts suggest an alternate strategy for structure-based inhibitor design.
[[Category: Caldanaerobacter subterraneus subsp. tengcongensis]]
 
[[Category: Large Structures]]
==About this Structure==
[[Category: Chen Q]]
2GEB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacter_tengcongensis Thermoanaerobacter tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEB OCA].
[[Category: Luo M]]
 
==Reference==
Crystal structure of Thermoanaerobacter tengcongensis hypoxanthine-guanine phosphoribosyl transferase L160I mutant--insights into inhibitor design., Chen Q, You D, Liang Y, Su X, Gu X, Luo M, Zheng X, FEBS J. 2007 Sep;274(17):4408-15. Epub 2007 Jul 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17662107 17662107]
[[Category: Hypoxanthine phosphoribosyltransferase]]
[[Category: Single protein]]
[[Category: Thermoanaerobacter tengcongensis]]
[[Category: Chen, Q.]]
[[Category: Luo, M.]]
[[Category: hgprt]]
[[Category: inhibitor design]]
[[Category: mutant]]
[[Category: selectivity]]
[[Category: transferase]]
 
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