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[[Image:2gej.jpg|left|200px]]


{{Structure
==Crystal Structure of phosphatidylinositol mannosyltransferase (PimA) from Mycobacterium smegmatis in complex with GDP-Man==
|PDB= 2gej |SIZE=350|CAPTION= <scene name='initialview01'>2gej</scene>, resolution 2.600&Aring;
<StructureSection load='2gej' size='340' side='right'caption='[[2gej]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=GDD:GUANOSINE-5&#39;-DIPHOSPHATE-ALPHA-D-MANNOSE'>GDD</scene>
<table><tr><td colspan='2'>[[2gej]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GEJ FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
|GENE= PIMA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1772 Mycobacterium smegmatis])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDD:GUANOSINE-5-DIPHOSPHATE-ALPHA-D-MANNOSE'>GDD</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gej OCA], [https://pdbe.org/2gej PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gej RCSB], [https://www.ebi.ac.uk/pdbsum/2gej PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gej ProSAT]</span></td></tr>
|RELATEDENTRY=[[2gek|2GEK]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gej OCA], [http://www.ebi.ac.uk/pdbsum/2gej PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gej RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/PIMA_MYCS2 PIMA_MYCS2] Catalyzes the addition of a mannose residue from GDP-D-mannose to the position 2 of a phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Contrary to PimB, the mannosyltransferase PimA is unable to transfer a mannose residue to the position 6 of the phosphatidyl-myo-inositola of PIM1.<ref>PMID:12068013</ref> <ref>PMID:19638342</ref>
 
== Evolutionary Conservation ==
'''Crystal Structure of phosphatidylinositol mannosyltransferase (PimA) from Mycobacterium smegmatis in complex with GDP-Man'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ge/2gej_consurf.spt"</scriptWhenChecked>
Phosphatidylinositol mannosyltransferase (PimA) is an essential enzyme for mycobacterial growth that catalyses the first mannosylation step in phosphatidyl-myo-inositol mannoside (PIM) biosynthesis. The enzyme belongs to the large GT4 family of glycosyltransferases, for which no structure is currently available. Recombinant purified PimA from Mycobacterium smegmatis has been crystallized in the presence of GDP and myo-inositol. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 37.2, b = 72.4, c = 138.2 A, and diffract to 2.4 A resolution.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
2GEJ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEJ OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gej ConSurf].
 
<div style="clear:both"></div>
==Reference==
== References ==
Crystallization and preliminary crystallographic analysis of PimA, an essential mannosyltransferase from Mycobacterium smegmatis., Guerin ME, Buschiazzo A, Kordulakova J, Jackson M, Alzari PM, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 May 1;61(Pt, 5):518-20. Epub 2005 Apr 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511084 16511084]
<references/>
[[Category: Mycobacterium smegmatis]]
__TOC__
[[Category: Protein complex]]
</StructureSection>
[[Category: Alzari, P M.]]
[[Category: Large Structures]]
[[Category: Buschiazzo, A.]]
[[Category: Mycolicibacterium smegmatis MC2 155]]
[[Category: Guerin, M E.]]
[[Category: Alzari PM]]
[[Category: Jackson, M.]]
[[Category: Buschiazzo A]]
[[Category: Kordulakova, J.]]
[[Category: Guerin ME]]
[[Category: binary complex,]]
[[Category: Jackson M]]
[[Category: gt4 glycosyltransferase]]
[[Category: Kordulakova J]]
[[Category: mannosyltransferase]]
[[Category: rossmann fold]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:15:33 2008''

Latest revision as of 09:27, 14 February 2024

Crystal Structure of phosphatidylinositol mannosyltransferase (PimA) from Mycobacterium smegmatis in complex with GDP-Man

2gej, resolution 2.60Å

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