1vrr

Crystal structure of the restriction endonuclease BstYI complex with DNA

spinqualitylabelsall models 1vrr, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1vrr is a 4 chain structure with sequence from Geobacillus stearothermophilus. This structure supersedes the now removed PDB entry 1yuv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.7Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q84AF2_GEOSE Publication Abstract from PubMed The type II restriction endonuclease BstYI recognizes the degenerate sequence 5'-RGATCY-3' (where R = A/G and Y = C/T), which overlaps with both BamHI (GGATCC) and BglII (AGATCT), and thus raises the question of whether BstYI DNA recognition will be more BamHI-like or BglII-like. We present here the structure of BstYI bound to a cognate DNA sequence (AGATCT). We find the complex to be more BglII-like with similarities mapping to DNA conformation, domain organization, and residues involved in catalysis. However, BstYI is unique in containing an extended arm subdomain, and the mechanism of DNA capture has both BglII-like and BamHI-like elements. Further, DNA recognition is more minimal than BglII and BamHI, where only two residues mediate recognition of the entire core sequence. Taken together, the structure reveals a mechanism of degenerate DNA recognition and offers insights into the possibilities and limitations in altering specificities of closely related restriction enzymes. Implications for switching restriction enzyme specificities from the structure of BstYI bound to a BglII DNA sequence.,Townson SA, Samuelson JC, Xu SY, Aggarwal AK Structure. 2005 May;13(5):791-801. PMID:15893669 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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