Chaperonin: Difference between revisions

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<StructureSection load='' size='350' side='right' caption='E. coli GroEL (green)/GroES (magenta) complex with ADP, AlF3, Mg+2 and K+ ions (PDB entry [[1pcq]])' scene='44/445432/Cv/1'>
<StructureSection load='' size='350' side='right' caption='E. coli GroEL (green)/GroES (magenta) complex with ADP, AlF3, Mg+2 and K+ ions (PDB entry [[1pcq]])' scene='44/445432/Cv/1'>
[[Image:1pcq.png|left|200px|thumb|Crystal Structure of Chaperonin, [[1pcq]]]]
[[Image:1pcq.png|left|200px|thumb|Crystal Structure of Chaperonin, [[1pcq]]]]
'''Chaperonins''' (CPN) are oligomeric proteins that mediate the folding of polypeptide chains.  '''Group I CPN''' are found in bacteria, chloroplasts and mitochondria.  For an introductory overview, see [http://en.wikipedia.org/wiki/Chaperonins Chaperonins in Wikipedia].
'''Chaperonins''' (Cpn) are oligomeric proteins that mediate the folding of polypeptide chains.  '''Group I CPN''' are found in bacteria, chloroplasts and mitochondria.  For an introductory overview, see [http://en.wikipedia.org/wiki/Chaperonins Chaperonins in Wikipedia].


The most characterized CPN are in the GroEL/GroES complex from ''Escherichia coli'' and CPN60/CPN10 from ''Thermus thermophilus''.<ref>PMID:18987317</ref>  The larger subunit (GroEL, CPN60) contains 3 domains: apical, intermediate and equatorial domain.  The apical domain is the one which binds the polypeptide substrate. The equatorial domains binds the nucleotide.  '''Group II CPNs''' are found in eukaryotic cytosol and archaea.  '''Thermosome''' is a CPN complex found in archaea.<ref>PMID:9546398</ref>  '''CCT''' is a CPN complex found in eukarya.<ref>PMID:22503819</ref>
The most characterized Cpn are in the GroEL/GroES complex from ''Escherichia coli'' and Cpn60/Cpn10 from ''Thermus thermophilus''.<ref>PMID:18987317</ref>  The larger subunit (GroEL, Cpn60) contains 3 domains: apical, intermediate and equatorial domain.  The apical domain is the one which binds the polypeptide substrate. The equatorial domains binds the nucleotide.   
*'''Group II Cpns''' are found during mitosis in eukaryotic cytosol and archaea.   
*'''Thermosome''' is a Cpn complex found in archaea.<ref>PMID:9546398</ref>   
*'''CCT''' or '''TRiC''' is a Cpn complex found in eukarya believed to be involved in uncontrolled proliferation.<ref>PMID:22503819</ref>, <ref>PMID:32297209</ref>
*<scene name='44/445432/Cv/2'>E. coli GroEL/GroES complex</scene> (GroEL in green, GroES in magenta, PDB entry [[1pcq]]).
*<scene name='44/445432/Cv/2'>E. coli GroEL/GroES complex</scene> (GroEL in green, GroES in magenta, PDB entry [[1pcq]]).
*<scene name='44/445432/Cv/3'>GroEL/GroES complex with ADP, AlF3, Mg+2 and K+ ions</scene>.  
*<scene name='44/445432/Cv/3'>GroEL/GroES complex with ADP, AlF3, Mg+2 and K+ ions</scene>.  
*<scene name='44/445432/Cv/5'>K+ ion coordination site</scene>. GroEL in cyan.
*<scene name='44/445432/Cv/6'>AlF3 binding site</scene>.
*<scene name='44/445432/Cv/7'>Mg+2 ion coordination site</scene>.
*<scene name='44/445432/Cv/9'>ADP binding site</scene>.
*<scene name='44/445432/Cv/10'>Whole binding site</scene>.
*See also [[Chaperones]].<br />
*See also [[Chaperones]].<br />
*For GroEl in Hebrew see [[Sand box groel]].
*For GroEl in Hebrew see [[Sand box groel]].
</StructureSection>
 
== 3D Structures of Chaperonin ==
== 3D Structures of Chaperonin ==
[[Chaperonin 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
== Files for 3D printer ==
{{#tree:id=OrganizedByTopic|openlevels=0|
<i class="fas fa-cubes"></i> Asymmetric Chaperonin Complex GroEL/GroES by [[User:Marius Mihasan|Marius Mihasan]] [https://3dprint.nih.gov/discover/3dpx-017057  <i class="fas fa-download"></i>]


*'''Group I'''
</StructureSection>
 
*''Large subunit''
 
**[[3m6c]] – MtGroEL1 apical domain – ''Mycobacterium tuberculosis''<BR />
**[[1sjp]], [[3rtk]] – MtGroEL2 residues 42-539<BR />
**[[2eu1]], [[4v43]], [[1oel]], [[1grl]], [[2yey]], [[4wsc]], [[4wgl]] – EcGroEL (mutant) - ''Escherichia coli''<BR />
**[[3e76]], [[2nwc]], [[1xck]], [[1ss8]], [[4hel]] – EcGroEL<BR />
**[[3c9v]], [[3cau]], [[2ynj]], [[5w0s]] – EcGroEL – Cryo EM<BR />
**[[2c7e]], [[1gr5]], [[4aaq]] - EcGroEL (mutant) – Cryo EM<BR />
**[[1dk7]], [[1la1]] - EcGroEL apical domain<br />
**[[1fy9]], [[1fya]], [[1kid]], [[1jon]], [[3vz6]], [[3vz7]], [[3vz8]] – EcGroEL apical domain (mutant) <BR />
**[[1srv]] – TtCPN60 apical domain - ''Thermus thermophilus''<BR />
**[[3osx]] - CPN60 apical domain – ''Xenorhabdus nematophila''<br />
**[[1iok]] – CPN60 – ''Paracoccus denitrificans''<br />
**[[5da8]] - GroEL – ''Chlorobium tepidum''<br />
**[[5cdk]], [[5cdj]], [[5cdi]] - GroEL apical domain – ''Chlamydomonas reinhardtii''<br />
 
*''Large subunit binary complex''
 
**[[1kp8]] - EcGroEL (mutant) + ATP<BR />
**[[4aar]], [[4aas]], [[4aau]], [[4ab2]], [[4ab3]] - EcGroEL (mutant) + ATP – Cryo EM<br />
**[[1sx3]] - EcGroEL + ATP<BR />
**[[1sx4]] - EcGroEL + ADP<BR />
**[[4ki8]] - EcGroEL (mutant) + ADP<br />
**[[1mnf]] – EcGroEL + polypeptide<BR />
**[[2cgt]] – EcGroEL + capsid assbly protein GP31 – EM<BR />
**[[1dkd]] - EcGroEL apical domain + polypeptide<br />
**[[4pj1]] - GroEL + ADP – human<br />
 
*''Small subunit''
 
**[[3nx6]] – GroES residues 40-134 (mutant) – ''Xanthomonas oryzae''<BR />
**[[1wnr]] – TtCPN10 residues 1-94<BR />
**[[1p3h]], [[1hx5]] – MtCPN10<BR />
**[[1p82]], [[1p83]] - MtCPN10 residues 1-25 - NMR<BR />
**[[1egs]] - GroES residues 19-27 - NMR
 
*''Large + small subunit''
 
**[[1svt]], [[1pcq]] - EcGroEL + GroES<BR />
**[[1pf9]], [[1aon]], [[4pko]], [[5opx]] - EcGroEL + GroES + ADP<BR />
**[[3wvl]] - EcGroEL (mutant) + GroES + ATP<br />
**[[2c7c]], [[2c7d]] - EcGroEL + GroES – Cryo EM<BR />
**[[1gru]] - EcGroEL + GroES + ATP + ADP – Cryo EM<BR />
**[[3zpz]], [[3zq0]], [[3zq1]] - EcGroEL + GroES + ADP – Cryo EM<br />
**[[4v4o]], [[4pkn]] - TtCPN60 + CPN10 + ADP
 
*'''Group II'''
 
**[[3izh]], [[3izi]], [[3izj]], [[3izk]], [[3izl]], [[3izm]], [[3izn]], [[3los]], [[3iyf]], [[3j3x]] – MmCPN – ''Methanococcus maripaludis'' – EM<BR />
**[[3kfb]], [[3kfe]], [[3kfk]] – MmCPN<BR />
**[[3j02]], [[3j03]] – MmCPN (mutant) – Cryo EM<br />
**[[3ruq]] - MmCPN + ADP<br />
**[[3rus]] - MmCPN (mutant) + ADP<br />
**[[3ruw]] - MmCPN (mutant) + ADP-AlF3<br />
**[[3ruv]] - MmCPN (mutant) + ATP analog<br />
 
*'''CCT'''
 
**[[2xsm]], [[4b2t]] – bCPN CCT – bovine<BR />
**[[4v81]], [[4v94]], [[4v8r]] – CPN CCT - yeast<br />
**[[3iyg]], [[4a0o]] - bCPN CCT – Cryo EM<BR />
**[[4a0v]] - bCPN CCT + AMPPNP – Cryo EM<br />
**[[4a0w]] - bCPN CCT + ADP + AlF3 – Cryo EM<br />
**[[4a13]] - bCPN CCT + ADP – Cryo EM<br />
**[[3ktt]] - bCPN CCT β subunit – Cryo EM<BR />
**[[1gml]], [[1gn1]] - CPN CCT γ subunit apical domain – mouse
 
*'''Hsp33'''
 
See [[Heat Shock Proteins]]
 
*'''Thermosome'''
 
**[[1a6d]] - TaTherm α+β subunits – ''Thermoplasma acidophilum''<BR />
**[[1a6e]] - TaTherm α+β subunits + ADP<BR />
**[[1ass]], [[1asx]] - TaTherm α apical domain<BR />
**[[1e0r]] – TaTherm β apical domain<br />
**[[3ko1]] – AtTherm α subunit– ''Acidianus tengchongensis''<br />
**[[3j1b]], [[3j1c]], [[3j1e]] - AtTherm α subunit – Cryo EM <br />
**[[3j1f]] - AtTherm β subunit + ATP – Cryo EM <br />
**[[3aq1]] – Therm – ''Methanococcoides burtonii''<br />
**[[1q2v]], [[1q3r]] – TkTherm α subunit (mutant) – ''Thermococcus KS-1''<br />
**[[1q3q]] - TkTherm α subunit (mutant) + AMP-PNP<br />
**[[1q3s]] - TkTherm α subunit (mutant) + ADP<br />
**[[4xcd]] - SsTherm β subunit + ADP – ''Sulfolobus solfataricus''<br />
**[[4xcg]], [[4xci]] - SsTherm α+β subunits + ADP<br />
 
**[[1lep]] – CPN-10 – ''Mycobacterium leprae''
 
*'''Group III'''
 
*''Thermosome''
 
**[[5x9u]] - ChTherm α subunit + ADP – ''Carboxydothermus hydrogenoformans''<br />
**[[5x9v]] - ChTherm α subunit + ANP <br />


}}
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]
[[Category:3D printer files]]
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