6cww: Difference between revisions
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New page: '''Unreleased structure''' The entry 6cww is ON HOLD Authors: Kearney, C., Olenginski, L.T., Hirn, T.D., Fowler, G.D., Tariq, D., Brewer, S.H., Phillips-Piro, C.M. Description: Cs H-NO... |
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==Cs H-NOX mutant with unnatural amino acid 4-cyano-L-phenylalanine at site 5== | |||
<StructureSection load='6cww' size='340' side='right'caption='[[6cww]], [[Resolution|resolution]] 1.85Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6cww]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus Caldanaerobacter subterraneus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CWW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CWW FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.851Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=211:2,2,2-NITRILOTRIETHANOL'>211</scene>, <scene name='pdbligand=4CF:4-CYANO-L-PHENYLALANINE'>4CF</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cww OCA], [https://pdbe.org/6cww PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cww RCSB], [https://www.ebi.ac.uk/pdbsum/6cww PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cww ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8RBX6_CALS4 Q8RBX6_CALS4] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The vibrational reporter unnatural amino acid (UAA) 4-cyano-l-phenylalanine (pCNF) was genetically incorporated individually at three sites (5, 36, and 78) in the heme protein Caldanaerobacter subterraneus H-NOX to probe local hydration environments. The UAA pCNF was incorporated site-specifically using an engineered, orthogonal tRNA synthetase in E. coli. The ability of all of the pCNF-containing H-NOX proteins to form the ferrous CO, NO, or O2 ligated and unligated states was confirmed with UV-Vis spectroscopy. The solvation state at each site of the three sites of pCNF incorporation was assessed using temperature-dependent infrared spectroscopy. Specifically, the frequency-temperature line slope (FTLS) method was utilized to show that the nitrile group at site 36 was fully solvated and the nitrile group at site 78 was de-solvated (buried) in the heme pocket. The nitrile group at site 5 was found to be partially solvated suggesting that the nitrile group was involved in moderate strength hydrogen bonds. These results were confirmed by the determination of the X-ray crystal structure of the H-NOX protein construct containing pCNF at site 5. | |||
Exploring local solvation environments of a heme protein using the spectroscopic reporter 4-cyano-l-phenylalanine.,Kearney C, Olenginski LT, Hirn TD, Fowler GD, Tariq D, Brewer SH, Phillips-Piro CM RSC Adv. 2018 Apr 9;8(24):13503-13512. doi: 10.1039/c8ra02000k. Epub 2018 Apr 10. PMID:29780583<ref>PMID:29780583</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6cww" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: Kearney | *[[Chemotaxis protein 3D structures|Chemotaxis protein 3D structures]] | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Caldanaerobacter subterraneus]] | |||
[[Category: Large Structures]] | |||
[[Category: Brewer SH]] | |||
[[Category: Fowler GD]] | |||
[[Category: Hirn TD]] | |||
[[Category: Kearney C]] | |||
[[Category: Olenginski LT]] | |||
[[Category: Phillips-Piro CM]] | |||
[[Category: Tariq D]] | |||
Latest revision as of 15:12, 4 October 2023
Cs H-NOX mutant with unnatural amino acid 4-cyano-L-phenylalanine at site 5
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