6g6m: Difference between revisions

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New page: '''Unreleased structure''' The entry 6g6m is ON HOLD Authors: Noguchi, H., Addy, C., Simoncini, D., Van Meervelt, L., Schiex, T., Zhang, K.Y.J., Tame, J.R.H., Voet, A.R.D. Description:...
 
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'''Unreleased structure'''


The entry 6g6m is ON HOLD
==Crystal structure of the computationally designed Tako8 protein in P42212==
<StructureSection load='6g6m' size='340' side='right'caption='[[6g6m]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6g6m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6G6M FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6g6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g6m OCA], [https://pdbe.org/6g6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6g6m RCSB], [https://www.ebi.ac.uk/pdbsum/6g6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6g6m ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
beta-Propeller proteins form one of the largest families of protein structures, with a pseudo-symmetrical fold made up of subdomains called blades. They are not only abundant but are also involved in a wide variety of cellular processes, often by acting as a platform for the assembly of protein complexes. WD40 proteins are a subfamily of propeller proteins with no intrinsic enzymatic activity, but their stable, modular architecture and versatile surface have allowed evolution to adapt them to many vital roles. By computationally reverse-engineering the duplication, fusion and diversification events in the evolutionary history of a WD40 protein, a perfectly symmetrical homologue called Tako8 was made. If two or four blades of Tako8 are expressed as single polypeptides, they do not self-assemble to complete the eight-bladed architecture, which may be owing to the closely spaced negative charges inside the ring. A different computational approach was employed to redesign Tako8 to create Ika8, a fourfold-symmetrical protein in which neighbouring blades carry compensating charges. Ika2 and Ika4, carrying two or four blades per subunit, respectively, were found to assemble spontaneously into a complete eight-bladed ring in solution. These artificial eight-bladed rings may find applications in bionanotechnology and as models to study the folding and evolution of WD40 proteins.


Authors: Noguchi, H., Addy, C., Simoncini, D., Van Meervelt, L., Schiex, T., Zhang, K.Y.J., Tame, J.R.H., Voet, A.R.D.
Computational design of symmetrical eight-bladed beta-propeller proteins.,Noguchi H, Addy C, Simoncini D, Wouters S, Mylemans B, Van Meervelt L, Schiex T, Zhang KYJ, Tame JRH, Voet ARD IUCrJ. 2019 Jan 1;6(Pt 1):46-55. doi: 10.1107/S205225251801480X. eCollection 2019, Jan 1. PMID:30713702<ref>PMID:30713702</ref>


Description: Crystal structure of the computationally designed Tako8 protein in P42212
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Schiex, T]]
<div class="pdbe-citations 6g6m" style="background-color:#fffaf0;"></div>
[[Category: Van Meervelt, L]]
== References ==
[[Category: Zhang, K.Y.J]]
<references/>
[[Category: Addy, C]]
__TOC__
[[Category: Noguchi, H]]
</StructureSection>
[[Category: Tame, J.R.H]]
[[Category: Large Structures]]
[[Category: Voet, A.R.D]]
[[Category: Synthetic construct]]
[[Category: Simoncini, D]]
[[Category: Addy C]]
[[Category: Noguchi H]]
[[Category: Schiex T]]
[[Category: Simoncini D]]
[[Category: Tame JRH]]
[[Category: Van Meervelt L]]
[[Category: Voet ARD]]
[[Category: Zhang KYJ]]
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