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==Crystal structure of Lymphocytic choriomeningitis mammarenavirus endonuclease bound to compound L742001==
==Crystal structure of Lymphocytic choriomeningitis mammarenavirus endonuclease bound to compound L742001==
<StructureSection load='5t2t' size='340' side='right' caption='[[5t2t]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
<StructureSection load='5t2t' size='340' side='right'caption='[[5t2t]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5t2t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lcmv Lcmv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T2T FirstGlance]. <br>
<table><tr><td colspan='2'>[[5t2t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lymphocytic_choriomeningitis_mammarenavirus Lymphocytic choriomeningitis mammarenavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T2T FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0N8:(2Z)-4-[1-BENZYL-4-(4-CHLOROBENZYL)PIPERIDIN-4-YL]-2-HYDROXY-4-OXOBUT-2-ENOIC+ACID'>0N8</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.967&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">L, KUE_IGS310002 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11623 LCMV])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0N8:(2Z)-4-[1-BENZYL-4-(4-CHLOROBENZYL)PIPERIDIN-4-YL]-2-HYDROXY-4-OXOBUT-2-ENOIC+ACID'>0N8</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t2t OCA], [https://pdbe.org/5t2t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t2t RCSB], [https://www.ebi.ac.uk/pdbsum/5t2t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t2t ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t2t OCA], [http://pdbe.org/5t2t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t2t RCSB], [http://www.ebi.ac.uk/pdbsum/5t2t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t2t ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/A0A059U382_9VIRU A0A059U382_9VIRU]] RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner.[PIRNR:PIRNR000836]
[https://www.uniprot.org/uniprot/L_LYCVA L_LYCVA] RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner.<ref>PMID:7682625</ref>


==See Also==
==See Also==
*[[RNA polymerase|RNA polymerase]]
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Lcmv]]
[[Category: Large Structures]]
[[Category: RNA-directed RNA polymerase]]
[[Category: Lymphocytic choriomeningitis mammarenavirus]]
[[Category: Alvarez, K]]
[[Category: Alvarez K]]
[[Category: Canard, B]]
[[Category: Canard B]]
[[Category: Ferron, F]]
[[Category: Ferron F]]
[[Category: Saez-Ayala, M]]
[[Category: Saez-Ayala M]]
[[Category: Yekwa, E L]]
[[Category: Yekwa EL]]
[[Category: Endonuclease]]
[[Category: Inhibitor]]
[[Category: Mammarenavirus]]
[[Category: Transferase]]
Retrieved from "https://proteopedia.org/5t2t"