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| [[Image:2hqo.jpg|left|200px]]
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| {{Structure
| | ==Structure of a Atypical Orphan Response Regulator Protein Revealed a New Phosphorylation-Independent Regulatory Mechanism== |
| |PDB= 2hqo |SIZE=350|CAPTION= <scene name='initialview01'>2hqo</scene>
| | <StructureSection load='2hqo' size='340' side='right'caption='[[2hqo]]' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND=
| | <table><tr><td colspan='2'>[[2hqo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_J99 Helicobacter pylori J99]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HQO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HQO FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| |GENE=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hqo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hqo OCA], [https://pdbe.org/2hqo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hqo RCSB], [https://www.ebi.ac.uk/pdbsum/2hqo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hqo ProSAT]</span></td></tr> |
| |DOMAIN=
| | </table> |
| |RELATEDENTRY=[[2hqn|2HQN]], [[2hqr|2HQR]]
| | == Function == |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hqo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hqo OCA], [http://www.ebi.ac.uk/pdbsum/2hqo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hqo RCSB]</span>
| | [https://www.uniprot.org/uniprot/Q9ZM42_HELPJ Q9ZM42_HELPJ] |
| }}
| | == Evolutionary Conservation == |
| | | [[Image:Consurf_key_small.gif|200px|right]] |
| '''Structure of a Atypical Orphan Response Regulator Protein Revealed a New Phosphorylation-Independent Regulatory Mechanism'''
| | Check<jmol> |
| | | <jmolCheckbox> |
| | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hq/2hqo_consurf.spt"</scriptWhenChecked> |
| ==Overview== | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| Two-component signal transduction systems, commonly found in prokaryotes, typically regulate cellular functions in response to environmental conditions through a phosphorylation-dependent process. A new type of response regulator, hp1043 (HP-RR) from Helicobacter pylori, has been recently identified. HP-RR is essential for cell growth and does not require the well known phosphorelay scheme. Unphosphorylated HP-RR binds specifically to its own promoter (P(1043)) and autoregulates the promoter of the tlpB gene (P(tlpB)). We have determined the structure of HP-RR by NMR and x-ray crystallography, revealing a symmetrical dimer with two functional domains. The molecular topology resembles that of the OmpR/PhoB subfamily, however, the symmetrical dimer is stable even in the unphosphorylated state. The dimer interface, formed by three secondary structure elements (alpha4-beta5-alpha5), resembles that of the active, phosphorylated forms of ArcA and PhoB. Several conserved residues of the HP-RR dimeric interface deviate from the OmpR/PhoB subfamily, although there are similar salt bridges and hydrophobic patches within the interface. Our findings reveal how a new type of response regulator protein could function as a cell growth-associated regulator in the absence of post-translational modification.
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | | </jmolCheckbox> |
| ==About this Structure== | | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hqo ConSurf]. |
| 2HQO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HQO OCA].
| | <div style="clear:both"></div> |
| | | __TOC__ |
| ==Reference==
| | </StructureSection> |
| Structure of an atypical orphan response regulator protein supports a new phosphorylation-independent regulatory mechanism., Hong E, Lee HM, Ko H, Kim DU, Jeon BY, Jung J, Shin J, Lee SA, Kim Y, Jeon YH, Cheong C, Cho HS, Lee W, J Biol Chem. 2007 Jul 13;282(28):20667-75. Epub 2007 May 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17491010 17491010]
| | [[Category: Helicobacter pylori J99]] |
| [[Category: Helicobacter pylori]] | | [[Category: Large Structures]] |
| [[Category: Single protein]] | | [[Category: Hong E]] |
| [[Category: Hong, E.]] | | [[Category: Lee W]] |
| [[Category: Lee, W.]] | |
| [[Category: phosporylation-independent response regulator]]
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| [[Category: regulatory domain]]
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| [[Category: symmetric dimer]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:34:02 2008''
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