6fq4: Difference between revisions

Latest revision as of 08:15, 14 June 2018

Structure of Chlamydial virulence factor TarP and vinculin head domain

Structural highlights

6fq4 is a 2 chain structure with sequence from Chick. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	VCL, VINC1 (CHICK)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[VINC_CHICK] Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Vinculin is a central component of mechanosensitive adhesive complexes that form between cells and the extracellular matrix. A myriad of infectious agents mimic vinculin binding sites (VBS), enabling them to hijack the adhesion machinery and facilitate cellular entry. Here, we report the structural and biochemical characterisation of a VBS from the chlamydial virulence factor TarP. Whilst the affinities of isolated VBS peptides from TarP and talin for vinculin are similar, their behaviour in larger fragments is markedly different. In talin, VBS are cryptic and require mechanical activation to bind vinculin, whereas the TarP VBS are located in disordered regions, and so are constitutively active. We demonstrate that the TarP VBS can uncouple talin:vinculin complexes, which may lead to adhesion destabilisation. This article is protected by copyright. All rights reserved.

Chlamydial virulence factor TarP mimics talin to disrupt the talin-vinculin complex.,Whitewood A, Singh AK, Brown DG, Goult BT FEBS Lett. 2018 Apr 30. doi: 10.1002/1873-3468.13074. PMID:29710402^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhang Z, Izaguirre G, Lin SY, Lee HY, Schaefer E, Haimovich B. The phosphorylation of vinculin on tyrosine residues 100 and 1065, mediated by SRC kinases, affects cell spreading. Mol Biol Cell. 2004 Sep;15(9):4234-47. Epub 2004 Jun 30. PMID:15229287 doi:10.1091/mbc.E04-03-0264
↑ le Duc Q, Shi Q, Blonk I, Sonnenberg A, Wang N, Leckband D, de Rooij J. Vinculin potentiates E-cadherin mechanosensing and is recruited to actin-anchored sites within adherens junctions in a myosin II-dependent manner. J Cell Biol. 2010 Jun 28;189(7):1107-15. doi: 10.1083/jcb.201001149. PMID:20584916 doi:10.1083/jcb.201001149
↑ Peng X, Cuff LE, Lawton CD, DeMali KA. Vinculin regulates cell-surface E-cadherin expression by binding to beta-catenin. J Cell Sci. 2010 Feb 15;123(Pt 4):567-77. doi: 10.1242/jcs.056432. Epub 2010 Jan , 19. PMID:20086044 doi:10.1242/jcs.056432
↑ Whitewood A, Singh AK, Brown DG, Goult BT. Chlamydial virulence factor TarP mimics talin to disrupt the talin-vinculin complex. FEBS Lett. 2018 Apr 30. doi: 10.1002/1873-3468.13074. PMID:29710402 doi:https://dx.doi.org/10.1002/1873-3468.13074

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Zhang Z, Izaguirre G, Lin SY, Lee HY, Schaefer E, Haimovich B. The phosphorylation of vinculin on tyrosine residues 100 and 1065, mediated by SRC kinases, affects cell spreading. Mol Biol Cell. 2004 Sep;15(9):4234-47. Epub 2004 Jun 30. PMID:15229287 doi:10.1091/mbc.E04-03-0264

[2] Duc Q, Shi Q, Blonk I, Sonnenberg A, Wang N, Leckband D, de Rooij J. Vinculin potentiates E-cadherin mechanosensing and is recruited to actin-anchored sites within adherens junctions in a myosin II-dependent manner. J Cell Biol. 2010 Jun 28;189(7):1107-15. doi: 10.1083/jcb.201001149. PMID:20584916 doi:10.1083/jcb.201001149

[3] Peng X, Cuff LE, Lawton CD, DeMali KA. Vinculin regulates cell-surface E-cadherin expression by binding to beta-catenin. J Cell Sci. 2010 Feb 15;123(Pt 4):567-77. doi: 10.1242/jcs.056432. Epub 2010 Jan , 19. PMID:20086044 doi:10.1242/jcs.056432

[4] Whitewood A, Singh AK, Brown DG, Goult BT. Chlamydial virulence factor TarP mimics talin to disrupt the talin-vinculin complex. FEBS Lett. 2018 Apr 30. doi: 10.1002/1873-3468.13074. PMID:29710402 doi:https://dx.doi.org/10.1002/1873-3468.13074

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@@ Line 3: / Line 3: @@
 <StructureSection load='6fq4' size='340' side='right' caption='[[6fq4]], [[Resolution|resolution]] 2.89&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6fq4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FQ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FQ4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6fq4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FQ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FQ4 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fq4 OCA], [http://pdbe.org/6fq4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fq4 RCSB], [http://www.ebi.ac.uk/pdbsum/6fq4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fq4 ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VCL, VINC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fq4 OCA], [http://pdbe.org/6fq4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fq4 RCSB], [http://www.ebi.ac.uk/pdbsum/6fq4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fq4 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Chick]]
 [[Category: Brown, D G]]
 [[Category: Goult, B T]]

6fq4: Difference between revisions

Latest revision as of 08:15, 14 June 2018

Structure of Chlamydial virulence factor TarP and vinculin head domain

Structural highlights

Function

Publication Abstract from PubMed

References

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