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==Crystal structure of a thermophilic O6-alkylguanine-DNA alkyltransferase-derived self-labeling protein-tag in covalent complex with SNAP-Vista Green==
==Crystal structure of a thermophilic O6-alkylguanine-DNA alkyltransferase-derived self-labeling protein-tag in covalent complex with SNAP-Vista Green==
<StructureSection load='6ga0' size='340' side='right' caption='[[6ga0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='6ga0' size='340' side='right'caption='[[6ga0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6ga0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GA0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GA0 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6ga0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GA0 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ETW:5-[(4-methylphenyl)methylcarbamoyl]-2-(6-oxidanyl-3-oxidanylidene-4~{H}-xanthen-9-yl)benzoic+acid'>ETW</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylated-DNA--[protein]-cysteine_S-methyltransferase Methylated-DNA--[protein]-cysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.63 2.1.1.63] </span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ETW:5-[(4-methylphenyl)methylcarbamoyl]-2-(3-oxidanyl-6-oxidanylidene-xanthen-9-yl)benzoic+acid'>ETW</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ga0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ga0 OCA], [http://pdbe.org/6ga0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ga0 RCSB], [http://www.ebi.ac.uk/pdbsum/6ga0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ga0 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ga0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ga0 OCA], [https://pdbe.org/6ga0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ga0 RCSB], [https://www.ebi.ac.uk/pdbsum/6ga0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ga0 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/OGT_SULSO OGT_SULSO]] Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.  
[https://www.uniprot.org/uniprot/OGT_SACS2 OGT_SACS2] Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.[HAMAP-Rule:MF_00772]
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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</div>
</div>
<div class="pdbe-citations 6ga0" style="background-color:#fffaf0;"></div>
<div class="pdbe-citations 6ga0" style="background-color:#fffaf0;"></div>
==See Also==
*[[DNA methyltransferase 3D structures|DNA methyltransferase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Miggiano, R]]
[[Category: Large Structures]]
[[Category: Rizzi, M]]
[[Category: Saccharolobus solfataricus P2]]
[[Category: Rossi, F]]
[[Category: Miggiano R]]
[[Category: Extremophile]]
[[Category: Rizzi M]]
[[Category: Fluorescent probe]]
[[Category: Rossi F]]
[[Category: O6-alkylguanine-dna alkyltransferase]]
[[Category: Protein engineering]]
[[Category: Self-labeling protein tag]]
[[Category: Transferase]]
Retrieved from "https://proteopedia.org/6ga0"