2i7x: Difference between revisions

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[[Image:2i7x.jpg|left|200px]]


{{Structure
==Structure of Yeast CPSF-100 (Ydh1p)==
|PDB= 2i7x |SIZE=350|CAPTION= <scene name='initialview01'>2i7x</scene>, resolution 2.50&Aring;
<StructureSection load='2i7x' size='340' side='right'caption='[[2i7x]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[2i7x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I7X FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
|GENE= CFT2, YDH1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i7x OCA], [https://pdbe.org/2i7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i7x RCSB], [https://www.ebi.ac.uk/pdbsum/2i7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i7x ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=[[2i7t|2I7T]], [[2i7v|2I7V]]
== Function ==
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i7x OCA], [http://www.ebi.ac.uk/pdbsum/2i7x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2i7x RCSB]</span>
[https://www.uniprot.org/uniprot/CFT2_YEAST CFT2_YEAST] RNA-binding component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. May be involved in poly(A)-site recognition. May be involved in the association of the CPF, CPFIA and RNA polymerase II complexes.<ref>PMID:11406596</ref> <ref>PMID:12853609</ref>
}}
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
'''Structure of Yeast CPSF-100 (Ydh1p)'''
Check<jmol>
 
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i7/2i7x_consurf.spt"</scriptWhenChecked>
==Overview==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
Most eukaryotic messenger RNA precursors (pre-mRNAs) undergo extensive maturational processing, including cleavage and polyadenylation at the 3'-end. Despite the characterization of many proteins that are required for the cleavage reaction, the identity of the endonuclease is not known. Recent analyses indicated that the 73-kDa subunit of cleavage and polyadenylation specificity factor (CPSF-73) might be the endonuclease for this and related reactions, although no direct data confirmed this. Here we report the crystal structures of human CPSF-73 at 2.1 A resolution, complexed with zinc ions and a sulphate that might mimic the phosphate group of the substrate, and the related yeast protein CPSF-100 (Ydh1) at 2.5 A resolution. Both CPSF-73 and CPSF-100 contain two domains, a metallo-beta-lactamase domain and a novel beta-CASP (named for metallo-beta-lactamase, CPSF, Artemis, Snm1, Pso2) domain. The active site of CPSF-73, with two zinc ions, is located at the interface of the two domains. Purified recombinant CPSF-73 possesses RNA endonuclease activity, and mutations that disrupt zinc binding in the active site abolish this activity. Our studies provide the first direct experimental evidence that CPSF-73 is the pre-mRNA 3'-end-processing endonuclease.
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==About this Structure==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i7x ConSurf].
2I7X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I7X OCA].  
<div style="clear:both"></div>
 
== References ==
==Reference==
<references/>
Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease., Mandel CR, Kaneko S, Zhang H, Gebauer D, Vethantham V, Manley JL, Tong L, Nature. 2006 Dec 14;444(7121):953-6. Epub 2006 Nov 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17128255 17128255]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Gebauer D]]
[[Category: Gebauer, D.]]
[[Category: Mandel CR]]
[[Category: Mandel, C R.]]
[[Category: Tong L]]
[[Category: Tong, L.]]
[[Category: Zhang H]]
[[Category: Zhang, H.]]
[[Category: artemi]]
[[Category: double-strand break repair]]
[[Category: metallo-b-lactamase]]
[[Category: polyadenylation]]
[[Category: pre-mrna processing]]
[[Category: v(d)j recombination]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:40:59 2008''

Latest revision as of 09:02, 21 February 2024

Structure of Yeast CPSF-100 (Ydh1p)

2i7x, resolution 2.50Å

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