6gix: Difference between revisions
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New page: '''Unreleased structure''' The entry 6gix is ON HOLD Authors: Palm, D.M., Agostini, A., Avaresch, V., Girr, P., Werwie, M., Takahashi, S., Satoh, H., Jaenicke, E., Paulsen, H. Descript... |
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==Water-soluble Chlorophyll Protein (WSCP) from Lepidium virginicum (Mutation L91P) with Chlorophyll-b== | |||
<StructureSection load='6gix' size='340' side='right'caption='[[6gix]], [[Resolution|resolution]] 2.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6gix]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lepidium_virginicum Lepidium virginicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GIX FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gix OCA], [https://pdbe.org/6gix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gix RCSB], [https://www.ebi.ac.uk/pdbsum/6gix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gix ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/O04797_LEPVR O04797_LEPVR] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We altered the chlorophyll (Chl) binding sites in various versions of water-soluble chlorophyll protein (WSCP) by amino acid exchanges to alter their preferences for either Chl a or Chl b. WSCP is ideally suited for this mutational analysis since it forms a tetrameric complex with only four identical Chl binding sites. A loop of 4-6 amino acids is responsible for Chl a versus Chl b selectivity. We show that a single amino acid exchange within this loop changes the relative Chl a/b affinities by a factor of 40. We obtained crystal structures of this WSCP variant binding either Chl a or Chl b. The Chl binding sites in these structures were compared with those in the major light-harvesting complex (LHCII) of the photosynthetic apparatus in plants to search for similar structural features involved in Chl a/b binding specificity. | |||
Chlorophyll a/b binding-specificity in water-soluble chlorophyll protein.,Palm DM, Agostini A, Averesch V, Girr P, Werwie M, Takahashi S, Satoh H, Jaenicke E, Paulsen H Nat Plants. 2018 Oct 8. pii: 10.1038/s41477-018-0273-z. doi:, 10.1038/s41477-018-0273-z. PMID:30297830<ref>PMID:30297830</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6gix" style="background-color:#fffaf0;"></div> | ||
[[Category: Agostini | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Palm | [[Category: Large Structures]] | ||
[[Category: | [[Category: Lepidium virginicum]] | ||
[[Category: | [[Category: Agostini A]] | ||
[[Category: | [[Category: Averesch V]] | ||
[[Category: Girr P]] | |||
[[Category: Jaenicke E]] | |||
[[Category: Palm DM]] | |||
[[Category: Paulsen H]] | |||
[[Category: Satoh H]] | |||
[[Category: Takahashi S]] | |||
[[Category: Werwie M]] | |||
Latest revision as of 12:41, 6 November 2024
Water-soluble Chlorophyll Protein (WSCP) from Lepidium virginicum (Mutation L91P) with Chlorophyll-b
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