6d9z: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:


==Structure of CysZ, a sulfate permease from Pseudomonas Denitrificans==
==Structure of CysZ, a sulfate permease from Pseudomonas Denitrificans==
<StructureSection load='6d9z' size='340' side='right' caption='[[6d9z]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
<StructureSection load='6d9z' size='340' side='right'caption='[[6d9z]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6d9z]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D9Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D9Z FirstGlance]. <br>
<table><tr><td colspan='2'>[[6d9z]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_denitrificans_(nom._rej.) Pseudomonas denitrificans (nom. rej.)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D9Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6D9Z FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4021318&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3tx3|3tx3]], [[6d79|6d79]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d9z OCA], [http://pdbe.org/6d9z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d9z RCSB], [http://www.ebi.ac.uk/pdbsum/6d9z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d9z ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6d9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d9z OCA], [https://pdbe.org/6d9z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6d9z RCSB], [https://www.ebi.ac.uk/pdbsum/6d9z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6d9z ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/M4XKU7_9PSED M4XKU7_9PSED]] High affinity, high specificity proton-dependent sulfate transporter, which mediates sulfate uptake. Provides the sulfur source for the cysteine synthesis pathway.[HAMAP-Rule:MF_00468][SAAS:SAAS00541081]  
[https://www.uniprot.org/uniprot/M4XKU7_9PSED M4XKU7_9PSED] High affinity, high specificity proton-dependent sulfate transporter, which mediates sulfate uptake. Provides the sulfur source for the cysteine synthesis pathway.[HAMAP-Rule:MF_00468][SAAS:SAAS00541081]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Sulfur, most abundantly found in the environment as sulfate (SO4(2-)), is an essential element in metabolites required by all living cells, including amino acids, co-factors and vitamins. However, current understanding of the cellular delivery of SO4(2-) at the molecular level is limited. CysZ has been described as a SO4(2-) permease, but its sequence family is without known structural precedent. Based on crystallographic structure information, SO4(2-) binding and flux experiments, we provide insight into the molecular mechanism of CysZ-mediated translocation of SO4(2-) across membranes. CysZ structures from three different bacterial species display a hitherto unknown fold and have subunits organized with inverted transmembrane topology. CysZ from Pseudomonas denitrificans assembles as a trimer of antiparallel dimers and the CysZ structures from two other species recapitulate dimers from this assembly. Mutational studies highlight the functional relevance of conserved CysZ residues.
 
Structure-based analysis of CysZ-mediated cellular uptake of sulfate.,Assur Sanghai Z, Liu Q, Clarke OB, Belcher-Dufrisne M, Wiriyasermkul P, Giese MH, Leal-Pinto E, Kloss B, Tabuso S, Love J, Punta M, Banerjee S, Rajashankar KR, Rost B, Logothetis D, Quick M, Hendrickson WA, Mancia F Elife. 2018 May 24;7. pii: 27829. doi: 10.7554/eLife.27829. PMID:29792261<ref>PMID:29792261</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6d9z" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Banerjee, S]]
[[Category: Large Structures]]
[[Category: Clarke, O B]]
[[Category: Banerjee S]]
[[Category: Hendrickson, W A]]
[[Category: Clarke OB]]
[[Category: Liu, Q]]
[[Category: Hendrickson WA]]
[[Category: Mancia, F]]
[[Category: Liu Q]]
[[Category: Rajashankar, K R]]
[[Category: Mancia F]]
[[Category: Sanghai, Z A]]
[[Category: Rajashankar KR]]
[[Category: Membrane protein]]
[[Category: Sanghai ZA]]
[[Category: Prokaryotic cysteine biosynthesis]]
[[Category: Sulfate translocation]]
[[Category: Transport protein]]
Retrieved from "https://proteopedia.org/6d9z"