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==Crystal structure of tetrameric restriction endonuclease SfiI in complex with cognate DNA (partial bound form)==
==Crystal structure of tetrameric restriction endonuclease SfiI in complex with cognate DNA (partial bound form)==
<StructureSection load='2f03' size='340' side='right' caption='[[2f03]], [[Resolution|resolution]] 3.05&Aring;' scene=''>
<StructureSection load='2f03' size='340' side='right'caption='[[2f03]], [[Resolution|resolution]] 3.05&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2f03]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F03 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F03 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2f03]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_fimbriatus Streptomyces fimbriatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F03 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F03 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.05&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ezv|2ezv]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f03 OCA], [https://pdbe.org/2f03 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f03 RCSB], [https://www.ebi.ac.uk/pdbsum/2f03 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f03 ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f03 OCA], [http://pdbe.org/2f03 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f03 RCSB], [http://www.ebi.ac.uk/pdbsum/2f03 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2f03 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/T2S1_STRFI T2S1_STRFI]] Recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves before N-9.  
[https://www.uniprot.org/uniprot/T2S1_STRFI T2S1_STRFI] Recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves before N-9.
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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==See Also==
==See Also==
*[[Endonuclease|Endonuclease]]
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Type II site-specific deoxyribonuclease]]
[[Category: Large Structures]]
[[Category: Aggarwal, A K]]
[[Category: Streptomyces fimbriatus]]
[[Category: Vanamee, E S]]
[[Category: Aggarwal AK]]
[[Category: Viadiu, H]]
[[Category: Vanamee ES]]
[[Category: Deoxyribonuclease]]
[[Category: Viadiu H]]
[[Category: Hydrolase-dna complex]]
[[Category: Protein-dna complex]]
[[Category: Type iif restriction endonuclease]]
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