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[[Image:2np6.gif|left|200px]]


{{Structure
==Crystal structure of the adenine-specific DNA methyltransferase M.TaqI complexed with the cofactor analog AETA and a 10 bp DNA containing an abasic site analog at the target position==
|PDB= 2np6 |SIZE=350|CAPTION= <scene name='initialview01'>2np6</scene>, resolution 2.10&Aring;
<StructureSection load='2np6' size='340' side='right'caption='[[2np6]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=3DR:1&#39;,2&#39;-DIDEOXYRIBOFURANOSE-5&#39;-PHOSPHATE'>3DR</scene>, <scene name='pdbligand=6MA:N6-METHYL-DEOXY-ADENOSINE-5&#39;-MONOPHOSPHATE'>6MA</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=NEA:5&#39;-DEOXY-5&#39;-[2-(AMINO)ETHYLTHIO]ADENOSINE'>NEA</scene>
<table><tr><td colspan='2'>[[2np6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NP6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NP6 FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
|GENE= taqIM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271 Thermus aquaticus])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3DR:1,2-DIDEOXYRIBOFURANOSE-5-PHOSPHATE'>3DR</scene>, <scene name='pdbligand=6MA:N6-METHYL-DEOXY-ADENOSINE-5-MONOPHOSPHATE'>6MA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=NEA:5-DEOXY-5-[2-(AMINO)ETHYLTHIO]ADENOSINE'>NEA</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2np6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2np6 OCA], [https://pdbe.org/2np6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2np6 RCSB], [https://www.ebi.ac.uk/pdbsum/2np6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2np6 ProSAT]</span></td></tr>
|RELATEDENTRY=[[1g38|1G38]], [[2adm|2ADM]], [[1aqi|1AQI]], [[1aqj|1AQJ]], [[2ih2|2IH2]], [[2ih4|2IH4]], [[2ih5|2IH5]], [[2np7|2NP7]], [[2ibs|2IBS]], [[2ibt|2IBT]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2np6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2np6 OCA], [http://www.ebi.ac.uk/pdbsum/2np6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2np6 RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/MTTA_THEAQ MTTA_THEAQ] This methylase recognizes the double-stranded sequence TCGA, causes specific methylation on A-4 on both strands and protects the DNA from cleavage by the TaqI endonuclease.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/2np6_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2np6 ConSurf].
<div style="clear:both"></div>


'''Crystal structure of the adenine-specific DNA methyltransferase M.TaqI complexed with the cofactor analog AETA and a 10 bp DNA containing an abasic site analog at the target position'''
==See Also==
 
*[[DNA methyltransferase 3D structures|DNA methyltransferase 3D structures]]
 
__TOC__
==About this Structure==
</StructureSection>
2NP6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NP6 OCA].
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Site-specific DNA-methyltransferase (adenine-specific)]]
[[Category: Thermus aquaticus]]
[[Category: Thermus aquaticus]]
[[Category: Lenz, T.]]
[[Category: Lenz T]]
[[Category: Scheidig, A J.]]
[[Category: Scheidig AJ]]
[[Category: Weinhold, E.]]
[[Category: Weinhold E]]
[[Category: abasic site analog]]
[[Category: base flipping]]
[[Category: dna]]
[[Category: dna methyltransferase]]
[[Category: nucleotide flipping]]
[[Category: transferase/dna complex]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:06:16 2008''
Retrieved from "https://proteopedia.org/2np6"