6a7y: Difference between revisions
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==Solution structure of an intermolecular leaped V-shape G-quadruplex== | |||
<StructureSection load='6a7y' size='340' side='right'caption='[[6a7y]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6a7y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sterkiella_nova Sterkiella nova]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A7Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A7Y FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a7y OCA], [https://pdbe.org/6a7y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a7y RCSB], [https://www.ebi.ac.uk/pdbsum/6a7y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a7y ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Aside from classical loops among G-quadruplexes, the unique leaped V-shape scaffold spans over three G-tetrads, without any intervening residues. This scaffold enables a sharp reversal of two adjacent strand directions and simultaneously participates in forming the G-tetrad core. These features make this scaffold itself distinctive and thus an essentially more accessible target. As an alternative to the conventional antisense method using a complementary chain, forming an intermolecular G-quadruplex from two different oligomers, in which the longer one as the target is captured by a short G-rich fragment, could be helpful for recognizing G-rich sequences and structural motifs. However, such an intermolecular leaped V-shape G-quadruplex consisting of DNA oligomers of quite different lengths has not been evaluated. Here, we present the first nuclear magnetic resonance (NMR) study of an asymmetric intermolecular leaped V-shape G-quadruplex assembled between an Oxytricha nova telomeric sequence d(G2T4G4T4G4) and a single G-tract fragment d(TG4A). Furthermore, we explored the selectivity of this short fragment as a potential probe, examined the kinetic discrimination for probing a specific mutant, and proposed the key sequence motif d(G2NG3NG4) essential for building the leaped V-shape G-quadruplexes. | |||
NMR solution structure of an asymmetric intermolecular leaped V-shape G-quadruplex: selective recognition of the d(G2NG3NG4) sequence motif by a short linear G-rich DNA probe.,Wan C, Fu W, Jing H, Zhang N Nucleic Acids Res. 2018 Nov 16. pii: 5184730. doi: 10.1093/nar/gky1167. PMID:30445650<ref>PMID:30445650</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6a7y" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Sterkiella nova]] | |||
[[Category: Wan CJ]] | |||
[[Category: Zhang N]] | |||
Latest revision as of 10:36, 14 June 2023
Solution structure of an intermolecular leaped V-shape G-quadruplex
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