6dzc: Difference between revisions
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New page: '''Unreleased structure''' The entry 6dzc is ON HOLD Authors: Sun, Y.-T., Varani, G. Description: Solution structure of Rbfox2 RRM mimetic peptide CPfox6 [[Category: Unreleased Structu... |
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The | ==Solution structure of Rbfox2 RRM mimetic peptide CPfox6== | ||
<StructureSection load='6dzc' size='340' side='right'caption='[[6dzc]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6dzc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fischeri Aspergillus fischeri]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DZC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DZC FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dzc OCA], [https://pdbe.org/6dzc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dzc RCSB], [https://www.ebi.ac.uk/pdbsum/6dzc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dzc ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The RNA recognition motif (RRM), which is the most abundant RNA-binding motif in eukaryotes, is a well-structured domain of about 90 amino acids, yet the beta2beta3 hairpin, corresponding to strands 2 and 3 of the beta-sheet, and the intervening loop make essential interactions with RNA in many RRM complexes. A series of small cyclic peptide mimics of the beta2beta3 hairpin of Rbfox2 protein that recognize the terminal loop of precursor miR-20b have been designed to investigate whether the full RNA-binding protein can be mimicked with a minimal structurally preorganized peptide. Within a small library of seven cyclic peptides, a peptide with low-micromolar affinity for the miR-20b precursor was found. NMR spectroscopy titration data suggest that this peptide specifically targets the apical loop of pre-miR-20b. This work shows that it is possible to mimic RNA-binding proteins with designed stable peptides, which provide a starting point for designing or evolving small peptide mimetics of RRM proteins. | |||
A Small Cyclic beta-Hairpin Peptide Mimics the Rbfox2 RNA Recognition Motif and Binds to the Precursor miRNA 20b.,Sun YT, Shortridge MD, Varani G Chembiochem. 2019 Apr 1;20(7):931-939. doi: 10.1002/cbic.201800645. Epub 2019 Feb, 15. PMID:30537200<ref>PMID:30537200</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Sun | <div class="pdbe-citations 6dzc" style="background-color:#fffaf0;"></div> | ||
[[Category: Varani | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Aspergillus fischeri]] | |||
[[Category: Large Structures]] | |||
[[Category: Sun Y-T]] | |||
[[Category: Varani G]] | |||
Latest revision as of 11:06, 30 October 2024
Solution structure of Rbfox2 RRM mimetic peptide CPfox6
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