6f2d: Difference between revisions

Latest revision as of 16:32, 9 July 2025

A FliPQR complex forms the core of the Salmonella type III secretion system export apparatus.

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Structural highlights

6f2d is a 10 chain structure with sequence from Salmonella enterica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 4.2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FLIP_SALTY Plays a role in the flagellum-specific transport system.

Publication Abstract from PubMed

Export of proteins through type III secretion systems is critical for motility and virulence of many major bacterial pathogens. Three putative integral membrane proteins (FliP, FliQ, FliR) are suggested to form the core of an export gate in the inner membrane, but their structure, assembly and location within the final nanomachine remain unclear. Here, we present the cryoelectron microscopy structure of the Salmonella Typhimurium FliP-FliQ-FliR complex at 4.2 A. None of the subunits adopt canonical integral membrane protein topologies, and common helix-turn-helix structural elements allow them to form a helical assembly with 5:4:1 stoichiometry. Fitting of the structure into reconstructions of intact secretion systems, combined with cross-linking, localize the export gate as a core component of the periplasmic portion of the machinery. This study thereby identifies the export gate as a key element of the secretion channel and implies that it primes the helical architecture of the components assembling downstream.

Structure of the core of the type III secretion system export apparatus.,Kuhlen L, Abrusci P, Johnson S, Gault J, Deme J, Caesar J, Dietsche T, Mebrhatu MT, Ganief T, Macek B, Wagner S, Robinson CV, Lea SM Nat Struct Mol Biol. 2018 Jul;25(7):583-590. doi: 10.1038/s41594-018-0086-9. Epub, 2018 Jul 2. PMID:29967543^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kuhlen L, Abrusci P, Johnson S, Gault J, Deme J, Caesar J, Dietsche T, Mebrhatu MT, Ganief T, Macek B, Wagner S, Robinson CV, Lea SM. Structure of the core of the type III secretion system export apparatus. Nat Struct Mol Biol. 2018 Jul;25(7):583-590. doi: 10.1038/s41594-018-0086-9. Epub, 2018 Jul 2. PMID:29967543 doi:https://dx.doi.org/10.1038/s41594-018-0086-9

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Kuhlen L, Abrusci P, Johnson S, Gault J, Deme J, Caesar J, Dietsche T, Mebrhatu MT, Ganief T, Macek B, Wagner S, Robinson CV, Lea SM. Structure of the core of the type III secretion system export apparatus. Nat Struct Mol Biol. 2018 Jul;25(7):583-590. doi: 10.1038/s41594-018-0086-9. Epub, 2018 Jul 2. PMID:29967543 doi:https://dx.doi.org/10.1038/s41594-018-0086-9

[1]

@@ Line 1: / Line 1: @@
 ==A FliPQR complex forms the core of the Salmonella type III secretion system export apparatus.==
-<StructureSection load='6f2d' size='340' side='right' caption='[[6f2d]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
+<SX load='6f2d' size='340' side='right' viewer='molstar' caption='[[6f2d]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6f2d]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cholerae-suis"_smith_1894 "bacillus cholerae-suis" smith 1894]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F2D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F2D FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6f2d]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F2D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6F2D FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fliP, LTSERUB_0568 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28901 "Bacillus cholerae-suis" Smith 1894]), fliR, flaP, STM1981 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28901 "Bacillus cholerae-suis" Smith 1894]), fliQ, flaQ, STY2188, t0897 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28901 "Bacillus cholerae-suis" Smith 1894])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.2&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f2d OCA], [http://pdbe.org/6f2d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f2d RCSB], [http://www.ebi.ac.uk/pdbsum/6f2d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f2d ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6f2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f2d OCA], [https://pdbe.org/6f2d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6f2d RCSB], [https://www.ebi.ac.uk/pdbsum/6f2d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6f2d ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/G5QE81_SALRU G5QE81_SALRU]] Plays a role in the flagellum-specific transport system.[RuleBase:RU362069] [[http://www.uniprot.org/uniprot/FLIQ_SALTI FLIQ_SALTI]] Required for the assembly of the rivet at the earliest stage of flagellar biosynthesis. [[http://www.uniprot.org/uniprot/FLIR_SALTY FLIR_SALTY]] Role in flagellar biosynthesis.
+[https://www.uniprot.org/uniprot/FLIP_SALTY FLIP_SALTY] Plays a role in the flagellum-specific transport system.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 18: / Line 18: @@
 </div>
 <div class="pdbe-citations 6f2d" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Flagellar protein 3D structures|Flagellar protein 3D structures]]
 == References ==
 <references/>
 __TOC__
-</StructureSection>
+</SX>
-[[Category: Bacillus cholerae-suis smith 1894]]
+[[Category: Large Structures]]
-[[Category: Abrusci, P]]
+[[Category: Salmonella enterica]]
-[[Category: Johnson, S]]
+[[Category: Abrusci P]]
-[[Category: Kuhlen, L]]
+[[Category: Johnson S]]
-[[Category: Lea, S M]]
+[[Category: Kuhlen L]]
-[[Category: Cryo-em]]
+[[Category: Lea SM]]
-[[Category: Export]]
-[[Category: Flagella]]
-[[Category: Membrane protein]]
-[[Category: Protein transport]]
-[[Category: T3ss]]

6f2d: Difference between revisions

Latest revision as of 16:32, 9 July 2025

A FliPQR complex forms the core of the Salmonella type III secretion system export apparatus.

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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