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[[Image:2okh.gif|left|200px]]


{{Structure
==Crystal structure of dimeric form of PfFabZ in crystal form3==
|PDB= 2okh |SIZE=350|CAPTION= <scene name='initialview01'>2okh</scene>, resolution 3.00&Aring;
<StructureSection load='2okh' size='340' side='right'caption='[[2okh]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[2okh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OKH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OKH FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
|GENE= fabZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 Plasmodium falciparum])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2okh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2okh OCA], [https://pdbe.org/2okh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2okh RCSB], [https://www.ebi.ac.uk/pdbsum/2okh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2okh ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=[[2oki|2OKI]], [[1zhg|1ZHG]], [[1z6b|1Z6B]], [[1u1z|1U1Z]]
== Function ==
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2okh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2okh OCA], [http://www.ebi.ac.uk/pdbsum/2okh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2okh RCSB]</span>
[https://www.uniprot.org/uniprot/Q965D7_PLAFA Q965D7_PLAFA]
}}
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ok/2okh_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2okh ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystals obtained from various batches of crystallization trials of FabZ from Plasmodium falciparum exhibited non-isomorphism. The c axis of the I222 cell showed a large variation of about 16 A, from c = 81 A to c = 97 A. Complete data sets were collected for three crystal forms with varying lengths of the c axis (form 1, c = 97 A; form 2, c = 92 A; form 3, c = 81 A). The crystal structure of form 1 has been reported previously. Here, the crystal structures of the other two crystal forms are reported and a detailed structural comparison is made of the three crystal forms in order to explore the possible reasons for the existence of non-isomorphism. The conformations of three loops vary between the three crystal forms. The disposition of the loops affects the crystal packing and hence the unit-cell parameter. The crystallization condition and crystallization method employed, which change the evaporation rate, determine the crystal form of the enzyme. The present analysis shows that pH-induced intrinsic conformational changes in the protein play a key role in the observed differences.


'''Crystal structure of dimeric form of PfFabZ in crystal form3'''
Packing and loop-structure variations in non-isomorphous crystals of FabZ from Plasmodium falciparum.,Swarnamukhi PL, Sharma SK, Padala P, Surolia N, Surolia A, Suguna K Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):458-64. Epub 2007, Mar 16. PMID:17372349<ref>PMID:17372349</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2okh" style="background-color:#fffaf0;"></div>


==Overview==
==See Also==
The crystals obtained from various batches of crystallization trials of FabZ from Plasmodium falciparum exhibited non-isomorphism. The c axis of the I222 cell showed a large variation of about 16 A, from c = 81 A to c = 97 A. Complete data sets were collected for three crystal forms with varying lengths of the c axis (form 1, c = 97 A; form 2, c = 92 A; form 3, c = 81 A). The crystal structure of form 1 has been reported previously. Here, the crystal structures of the other two crystal forms are reported and a detailed structural comparison is made of the three crystal forms in order to explore the possible reasons for the existence of non-isomorphism. The conformations of three loops vary between the three crystal forms. The disposition of the loops affects the crystal packing and hence the unit-cell parameter. The crystallization condition and crystallization method employed, which change the evaporation rate, determine the crystal form of the enzyme. The present analysis shows that pH-induced intrinsic conformational changes in the protein play a key role in the observed differences.
*[[Beta-hydroxyacyl-acyl carrier protein dehydratase 3D structures|Beta-hydroxyacyl-acyl carrier protein dehydratase 3D structures]]
 
== References ==
==About this Structure==
<references/>
2OKH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OKH OCA].
__TOC__
 
</StructureSection>
==Reference==
[[Category: Large Structures]]
Packing and loop-structure variations in non-isomorphous crystals of FabZ from Plasmodium falciparum., Swarnamukhi PL, Sharma SK, Padala P, Surolia N, Surolia A, Suguna K, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):458-64. Epub 2007, Mar 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17372349 17372349]
[[Category: Plasmodium falciparum]]
[[Category: Plasmodium falciparum]]
[[Category: Single protein]]
[[Category: Padala P]]
[[Category: Padala, P.]]
[[Category: Sharma SK]]
[[Category: Sharma, S K.]]
[[Category: Suguna K]]
[[Category: Suguna, K.]]
[[Category: Surolia A]]
[[Category: Surolia, A.]]
[[Category: Surolia N]]
[[Category: Surolia, N.]]
[[Category: Swarnamukhi PL]]
[[Category: Swarnamukhi, P L.]]
[[Category: fabz]]
[[Category: hotdog fold]]
[[Category: non-isomorphism]]
[[Category: plasmodium]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:19:08 2008''
Retrieved from "https://proteopedia.org/2okh"