2lpx: Difference between revisions

Latest revision as of 05:48, 15 May 2024

Solution Structure of Strawberry Allergen Fra a 1e

Structural highlights

2lpx is a 1 chain structure with sequence from Fragaria x ananassa. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FRA1E_FRAAN Involved in the control of flavonoid biosynthesis in fruits, probably by binding directly to natural flavonoids (PubMed:19969523). Binds the natural flavonoid quercetin-3-O-glucuronide with affinities in the low micromolar range (PubMed:24133217).^[1] ^[2]

Publication Abstract from PubMed

The PR10 family protein Fra a 1E from strawberry (Fragaria x ananassa) is down-regulated in white strawberry mutants, and transient RNAi (RNA interference)-mediated silencing experiments confirmed that Fra a 1 is involved in fruit pigment synthesis. In the present study, we determined the solution structure of Fra a 1E. The protein fold is identical with that of other members of the PR10 protein family and consists of a seven-stranded antiparallel beta-sheet, two short V-shaped alpha-helices and a long C-terminal alpha-helix that encompass a hydrophobic pocket. Whereas Fra a 1E contains the glycine-rich loop that is highly conserved throughout the protein family, the volume of the hydrophobic pocket and the size of its entrance are much larger than expected. The three-dimensional structure may shed some light on its physiological function and may help to further understand the role of PR10 proteins in plants.

Solution structure of the strawberry allergen Fra a 1.,Seutter von Loetzen C, Schweimer K, Schwab W, Rosch P, Hartl-Spiegelhauer O Biosci Rep. 2012 Dec 1;32(6):567-75. doi: 10.1042/BSR20120058. PMID:22913709^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Munoz C, Hoffmann T, Escobar NM, Ludemann F, Botella MA, Valpuesta V, Schwab W. The strawberry fruit Fra a allergen functions in flavonoid biosynthesis. Mol Plant. 2010 Jan;3(1):113-24. doi: 10.1093/mp/ssp087. Epub 2009 Oct 29. PMID:19969523 doi:https://dx.doi.org/10.1093/mp/ssp087
↑ Casanal A, Zander U, Munoz C, Dupeux F, Luque I, Botella MA, Schwab W, Valpuesta V, Marquez JA. The Strawberry Pathogenesis-Related 10 (PR-10) Fra a proteins control flavonoid biosynthesis by binding to metabolic intermediates. J Biol Chem. 2013 Oct 16. PMID:24133217 doi:https://dx.doi.org/10.1074/jbc.M113.501528
↑ Seutter von Loetzen C, Schweimer K, Schwab W, Rosch P, Hartl-Spiegelhauer O. Solution structure of the strawberry allergen Fra a 1. Biosci Rep. 2012 Dec 1;32(6):567-75. doi: 10.1042/BSR20120058. PMID:22913709 doi:https://dx.doi.org/10.1042/BSR20120058

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Munoz C, Hoffmann T, Escobar NM, Ludemann F, Botella MA, Valpuesta V, Schwab W. The strawberry fruit Fra a allergen functions in flavonoid biosynthesis. Mol Plant. 2010 Jan;3(1):113-24. doi: 10.1093/mp/ssp087. Epub 2009 Oct 29. PMID:19969523 doi:https://dx.doi.org/10.1093/mp/ssp087

[2] Casanal A, Zander U, Munoz C, Dupeux F, Luque I, Botella MA, Schwab W, Valpuesta V, Marquez JA. The Strawberry Pathogenesis-Related 10 (PR-10) Fra a proteins control flavonoid biosynthesis by binding to metabolic intermediates. J Biol Chem. 2013 Oct 16. PMID:24133217 doi:https://dx.doi.org/10.1074/jbc.M113.501528

[3] Seutter von Loetzen C, Schweimer K, Schwab W, Rosch P, Hartl-Spiegelhauer O. Solution structure of the strawberry allergen Fra a 1. Biosci Rep. 2012 Dec 1;32(6):567-75. doi: 10.1042/BSR20120058. PMID:22913709 doi:https://dx.doi.org/10.1042/BSR20120058

[1]

[2]

[3]

@@ Line 1: / Line 1: @@
 ==Solution Structure of Strawberry Allergen Fra a 1e==
-<StructureSection load='2lpx' size='340' side='right' caption='[[2lpx]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2lpx' size='340' side='right'caption='[[2lpx]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2lpx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fraan Fraan]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LPX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LPX FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2lpx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fragaria_x_ananassa Fragaria x ananassa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LPX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LPX FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FRAA1E.2, FRAA1E.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3747 FRAAN])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lpx OCA], [http://pdbe.org/2lpx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lpx RCSB], [http://www.ebi.ac.uk/pdbsum/2lpx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lpx ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lpx OCA], [https://pdbe.org/2lpx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lpx RCSB], [https://www.ebi.ac.uk/pdbsum/2lpx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lpx ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/FRA1E_FRAAN FRA1E_FRAAN] Involved in the control of flavonoid biosynthesis in fruits, probably by binding directly to natural flavonoids (PubMed:19969523). Binds the natural flavonoid quercetin-3-O-glucuronide with affinities in the low micromolar range (PubMed:24133217).<ref>PMID:19969523</ref> <ref>PMID:24133217</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Fraan]]
+[[Category: Fragaria x ananassa]]
-[[Category: Hartl-Spiegelhauer, O]]
+[[Category: Large Structures]]
-[[Category: Loetzen, C Seutter von]]
+[[Category: Hartl-Spiegelhauer O]]
-[[Category: Roesch, P]]
+[[Category: Roesch P]]
-[[Category: Schwab, W]]
+[[Category: Schwab W]]
-[[Category: Schweimer, K]]
+[[Category: Schweimer K]]
-[[Category: Bet v 1 homologous protein]]
+[[Category: Seutter von Loetzen C]]
-[[Category: Unknown function]]

2lpx: Difference between revisions

Latest revision as of 05:48, 15 May 2024

Solution Structure of Strawberry Allergen Fra a 1e

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools