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[[Image:2biw.gif|left|200px]]<br />
<applet load="2biw" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2biw, resolution 2.39&Aring;" />
'''CRYSTAL STRUCTURE OF APOCAROTENOID CLEAVAGE OXYGENASE FROM SYNECHOCYSTIS, NATIVE ENZYME'''<br />


==Overview==
==Crystal structure of apocarotenoid cleavage oxygenase from Synechocystis, native enzyme==
Enzymes that produce retinal and related apocarotenoids constitute a, sequence- and thus structure-related family, a member of which was, analyzed by x-ray diffraction. This member is an oxygenase and contains an, Fe2+-4-His arrangement at the axis of a seven-bladed beta-propeller chain, fold covered by a dome formed by six large loops. The Fe2+ is accessible, through a long nonpolar tunnel that holds a carotenoid derivative in one, of the crystals. On binding, three consecutive double bonds of this, carotenoid changed from a straight all-trans to a cranked cis-trans-cis, conformation. The remaining trans bond is located at the dioxygen-ligated, Fe2+ and cleaved by oxygen.
<StructureSection load='2biw' size='340' side='right'caption='[[2biw]], [[Resolution|resolution]] 2.39&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2biw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. The June 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Carotenoid Oxygenase''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_6 10.2210/rcsb_pdb/mom_2005_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BIW FirstGlance]. <br>
2BIW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.] with FE and 3ON as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 2BIW with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb66_1.html Carotenoid Oxygenase]]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BIW OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.39&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3ON:(3R)-3-HYDROXY-8-APOCAROTENOL'>3ON</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2biw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2biw OCA], [https://pdbe.org/2biw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2biw RCSB], [https://www.ebi.ac.uk/pdbsum/2biw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2biw ProSAT]</span></td></tr>
The structure of a retinal-forming carotenoid oxygenase., Kloer DP, Ruch S, Al-Babili S, Beyer P, Schulz GE, Science. 2005 Apr 8;308(5719):267-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15821095 15821095]
</table>
== Function ==
[https://www.uniprot.org/uniprot/ACOX_SYNY3 ACOX_SYNY3] Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta-carotene or lycopene.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bi/2biw_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2biw ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Carotenoid Oxygenase]]
[[Category: Carotenoid Oxygenase]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Synechocystis sp.]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Al-Babili, S.]]
[[Category: Synechocystis sp. PCC 6803]]
[[Category: Beyer, P.]]
[[Category: Al-Babili S]]
[[Category: Kloer, D.P.]]
[[Category: Beyer P]]
[[Category: Ruch, S.]]
[[Category: Kloer DP]]
[[Category: Schulz, G.E.]]
[[Category: Ruch S]]
[[Category: 3ON]]
[[Category: Schulz GE]]
[[Category: FE]]
[[Category: carotenoid cleavage]]
[[Category: dioxygenase]]
[[Category: non-heme iron]]
[[Category: oxidoreductase]]
[[Category: oxygenase]]
[[Category: retinal formation]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 17:40:13 2007''
Retrieved from "https://proteopedia.org/2biw"