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[[Image:2oqc.jpg|left|200px]]


{{Structure
==Crystal Structure of Penicillin V acylase from Bacillus subtilis==
|PDB= 2oqc |SIZE=350|CAPTION= <scene name='initialview01'>2oqc</scene>, resolution 2.50&Aring;
<StructureSection load='2oqc' size='340' side='right'caption='[[2oqc]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[2oqc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OQC FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
|GENE= yxeI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oqc OCA], [https://pdbe.org/2oqc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oqc RCSB], [https://www.ebi.ac.uk/pdbsum/2oqc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oqc ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=
== Function ==
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oqc OCA], [http://www.ebi.ac.uk/pdbsum/2oqc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2oqc RCSB]</span>
[https://www.uniprot.org/uniprot/YXEI_BACSU YXEI_BACSU]
}}
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oq/2oqc_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oqc ConSurf].
<div style="clear:both"></div>


'''Crystal Structure of Penicillin V acylase from Bacillus subtilis'''
==See Also==
 
*[[Penicillin acylase|Penicillin acylase]]
 
__TOC__
==Overview==
</StructureSection>
Penicillin acylase proteins are amidohydrolase enzymes that cleave penicillins at the amide bond connecting the side chain to their beta-lactam nucleus. An unannotated protein from Bacillus subtilis has been expressed in Escherichia coli, purified and confirmed to possess penicillin V acylase activity. The protein was crystallized using the hanging-drop vapour-diffusion method from a solution containing 4 M sodium formate in 100 mM Tris-HCl buffer pH 8.2. Diffraction data were collected under cryogenic conditions to a spacing of 2.5 A. The crystals belonged to the orthorhombic space group C222(1), with unit-cell parameters a = 111.0, b = 308.0, c = 56.0 A. The estimated Matthews coefficient was 3.23 A3 Da(-1), corresponding to 62% solvent content. The structure has been solved using molecular-replacement methods with B. sphaericus penicillin V acylase (PDB code 2pva) as the search model.
 
==About this Structure==
2OQC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQC OCA].
 
==Reference==
Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis., Rathinaswamy P, Pundle AV, Prabhune AA, Sivaraman H, Brannigan JA, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1;61(Pt, 7):680-3. Epub 2005 Jun 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511127 16511127]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Penicillin amidase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Brannigan JA]]
[[Category: Brannigan, J A.]]
[[Category: Dodson GG]]
[[Category: Dodson, G G.]]
[[Category: Prabhune AA]]
[[Category: Prabhune, A A.]]
[[Category: Pundle AV]]
[[Category: Pundle, A V.]]
[[Category: Rathinaswamy P]]
[[Category: Rathinaswamy, P.]]
[[Category: Sivaraman H]]
[[Category: Sivaraman, H.]]
[[Category: Suresh CG]]
[[Category: Suresh, C G.]]
[[Category: bacillus subtili]]
[[Category: choloylglycine hydrolase]]
[[Category: conjugated bile acid hydrolase]]
[[Category: ntn-hydrolase]]
[[Category: penicillin v acylase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:21:37 2008''

Latest revision as of 08:58, 25 October 2023

Crystal Structure of Penicillin V acylase from Bacillus subtilis

2oqc, resolution 2.50Å

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