Nitrate reductase

spinqualitylabelsall models Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo, NO3- and molybdepterin guanine nucleotide (PDB entry 2jiq) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Nitrate reductase (NR) catalyzes the reduction of NO3 to NO2 using NADPH. NR active site contains Mo atom. Four types of NR are known: eukaryotic assimilatory NR bacterial cytoplasmic assimilatory (NAC) bacterial membrane-bound respiratory (NAR) bacterial periplasmic dissimilatory (NAP). The cofactors of NR are Molybdopterin (MPT) in eukaryotic NR and bis-Molybdopterin guanine dinucleotide (MGD) in bacterial NR. Structural highlights Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo, NO3 and 2 molybdepterin guanine nucleotides. NAP active site includes a six-coordinated Mo atom which coordinates to 2 MGD molecules and Cys140. The NO3 molecule is guided to the active site by a funnel leading to the Mo atom.

3D structures of nitrate reductase

Updated on 23-July-2019

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• Bacterial periplasmic dissimilatory NR
  • 2nap, 2jim, 2jio, 2jip, 2v3v, 2v45 – DdNAP + MGD + Mo - Desulfovibrio desulfuricans
    
  • 2jiq - DdNAP + NO3 + MGD + Mo
    
  • 2jir - DdNAP + CN + MGD + Mo
    
  • 1jni – NAP small subunit + heme – Haemophilus influenzae
    
  • 1ogy - NAP + heme + MGD + Mo– Rhodobacter sphaeroides
    
  • 2nya - EcNAP + MGD + Mo – Escherichia coli
    
  • 3ml1, 3o5a - NAP catalytic subunit + NAPB + heme + MGD + Mo – Ralstonia eutropha
    
  • 5yly – NR cytochrome b5 reductase domain + FAD – Ulva prolifera
    
• Bacterial respiratory NR
  • 1q16, 1y5l – EcNAR α+β+γ chains + heme + MGD + Mo
    
  • 1r27 - EcNAR α+β chains + MGD + Mo
    
  • 1siw – EcNAR α+β+γ chains + heme
    
  • 1y4z, 3egw - EcNAR α+β (mutant) + γ chains + inhibitor + heme + MGD + Mo
    
  • 1y5i, 1y5n - EcNAR α+β + γ (mutant) chains + inhibitor + heme + MGD + Mo
    
  • 3ir5, 3ir7 - EcNAR α (mutant) +β + γ chains + inhibitor + heme + MGD + Mo
    
  • 3ir6 - EcNAR α (mutant) +β + γ chains + inhibitor + heme + GDP
• Eukaryotic assimilatory NR
  • 2bih, 2bii – NR MoCo-binding domain + MPT – Pichia angusta
• Eukaryotic NR
  • 1cne – cNR + FAD – corn
    
  • 1cnf, 2cnd – cNR (mutant) + FAD + ADP
    

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