2bkw: Difference between revisions
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== | ==Yeast alanine:glyoxylate aminotransferase YFL030w== | ||
<StructureSection load='2bkw' size='340' side='right'caption='[[2bkw]], [[Resolution|resolution]] 2.57Å' scene=''> | |||
== Structural highlights == | |||
[ | <table><tr><td colspan='2'>[[2bkw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BKW FirstGlance]. <br> | ||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.57Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLV:GLYOXYLIC+ACID'>GLV</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | |||
[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bkw OCA], [https://pdbe.org/2bkw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bkw RCSB], [https://www.ebi.ac.uk/pdbsum/2bkw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bkw ProSAT]</span></td></tr> | ||
</table> | |||
[[ | == Function == | ||
[https://www.uniprot.org/uniprot/AGX1_YEAST AGX1_YEAST] Has alanine:glyoxylate aminotransferase activity.<ref>PMID:3933486</ref> <ref>PMID:16226833</ref> | |||
== Evolutionary Conservation == | |||
[ | [[Image:Consurf_key_small.gif|200px|right]] | ||
[[ | Check<jmol> | ||
[ | <jmolCheckbox> | ||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/2bkw_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bkw ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We have determined the three-dimensional crystal structure of the protein encoded by the open reading frame YFL030w from Saccharomyces cerevisiae to a resolution of 2.6 A using single wavelength anomalous diffraction. YFL030w is a 385 amino-acid protein with sequence similarity to the aminotransferase family. The structure of the protein reveals a homodimer adopting the fold-type I of pyridoxal 5'-phosphate (PLP)-dependent aminotransferases. The PLP co-factor is covalently bound to the active site in the crystal structure. The protein shows close structural resemblance with the human alanine:glyoxylate aminotransferase (EC 2.6.1.44), an enzyme involved in the hereditary kidney stone disease primary hyperoxaluria type 1. In this paper we show that YFL030w codes for an alanine:glyoxylate aminotransferase, highly specific for its amino donor and acceptor substrates. | |||
Crystal structure and confirmation of the alanine:glyoxylate aminotransferase activity of the YFL030w yeast protein.,Meyer P, Liger D, Leulliot N, Quevillon-Cheruel S, Zhou CZ, Borel F, Ferrer JL, Poupon A, Janin J, van Tilbeurgh H Biochimie. 2005 Dec;87(12):1041-7. Epub 2005 Oct 5. PMID:16226833<ref>PMID:16226833</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2bkw" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Saccharomyces cerevisiae S288C]] | |||
[[Category: Borel F]] | |||
[[Category: Ferrer JL]] | |||
[[Category: Janin J]] | |||
[[Category: Leulliot N]] | |||
[[Category: Liger D]] | |||
[[Category: Meyer P]] | |||
[[Category: Poupon A]] | |||
[[Category: Quevillon-Cheruel S]] | |||
[[Category: Zhou CZ]] | |||
[[Category: Van Tilbeurgh H]] | |||
Latest revision as of 08:24, 9 April 2025
Yeast alanine:glyoxylate aminotransferase YFL030w
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