6ee8: Difference between revisions

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New page: '''Unreleased structure''' The entry 6ee8 is ON HOLD Authors: Darst, S.A., Campbell, E.A., Boyaci Selcuk, H., Chen, J. Description: Mycobacterium tuberculosis RNAP promoter unwinding i...
 
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'''Unreleased structure'''


The entry 6ee8 is ON HOLD
==Mycobacterium tuberculosis RNAP promoter unwinding intermediate complex with RbpA/CarD and AP3 promoter==
<SX load='6ee8' size='340' side='right' viewer='molstar' caption='[[6ee8]], [[Resolution|resolution]] 3.92&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ee8]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EE8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EE8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.92&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ee8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ee8 OCA], [https://pdbe.org/6ee8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ee8 RCSB], [https://www.ebi.ac.uk/pdbsum/6ee8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ee8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CARD_MYCTU CARD_MYCTU] Controls rRNA transcription by binding to the RNA polymerase (RNAP). Required for replication and persistence during infection of mice.<ref>PMID:19596241</ref> <ref>PMID:22904282</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A key regulated step of transcription is promoter melting by RNA polymerase (RNAP) to form the open promoter complex(1-3). To generate the open complex, the conserved catalytic core of the RNAP combines with initiation factors to locate promoter DNA, unwind 12-14 base pairs of the DNA duplex and load the template-strand DNA into the RNAP active site. Formation of the open complex is a multi-step process during which transient intermediates of unknown structure are formed(4-6). Here we present cryo-electron microscopy structures of bacterial RNAP-promoter DNA complexes, including structures of partially melted intermediates. The structures show that late steps of promoter melting occur within the RNAP cleft, delineate key roles for fork-loop 2 and switch 2-universal structural features of RNAP-in restricting access of DNA to the RNAP active site, and explain why clamp opening is required to allow entry of single-stranded template DNA into the active site. The key roles of fork-loop 2 and switch 2 suggest a common mechanism for late steps in promoter DNA opening to enable gene expression across all domains of life.


Authors: Darst, S.A., Campbell, E.A., Boyaci Selcuk, H., Chen, J.
Structures of an RNA polymerase promoter melting intermediate elucidate DNA unwinding.,Boyaci H, Chen J, Jansen R, Darst SA, Campbell EA Nature. 2019 Jan;565(7739):382-385. doi: 10.1038/s41586-018-0840-5. Epub 2019 Jan, 9. PMID:30626968<ref>PMID:30626968</ref>


Description: Mycobacterium tuberculosis RNAP promoter unwinding intermediate complex with RbpA/CarD and AP3 promoter
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Boyaci Selcuk, H]]
<div class="pdbe-citations 6ee8" style="background-color:#fffaf0;"></div>
[[Category: Chen, J]]
 
[[Category: Campbell, E.A]]
==See Also==
[[Category: Darst, S.A]]
*[[Sigma factor 3D structures|Sigma factor 3D structures]]
== References ==
<references/>
__TOC__
</SX>
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Boyaci Selcuk H]]
[[Category: Campbell EA]]
[[Category: Chen J]]
[[Category: Darst SA]]
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