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| [[Image:2p73.jpg|left|200px]]
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| {{Structure
| | ==crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1== |
| |PDB= 2p73 |SIZE=350|CAPTION= <scene name='initialview01'>2p73</scene>, resolution 2.300Å
| | <StructureSection load='2p73' size='340' side='right'caption='[[2p73]], [[Resolution|resolution]] 2.30Å' scene=''> |
| |SITE= | | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5'-DIPHOSPHATE'>UDP</scene>
| | <table><tr><td colspan='2'>[[2p73]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Paramecium_bursaria_Chlorella_virus_1 Paramecium bursaria Chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P73 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P73 FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| |GENE= A64R ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Paramecium bursaria Chlorella virus 1])
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr> |
| |DOMAIN=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p73 OCA], [https://pdbe.org/2p73 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p73 RCSB], [https://www.ebi.ac.uk/pdbsum/2p73 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p73 ProSAT]</span></td></tr> |
| |RELATEDENTRY=[[2p6w|2P6W]], [[2p72|2P72]]
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p73 OCA], [http://www.ebi.ac.uk/pdbsum/2p73 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2p73 RCSB]</span>
| | == Function == |
| }}
| | [https://www.uniprot.org/uniprot/Q89399_PBCV1 Q89399_PBCV1] |
| | | __TOC__ |
| '''crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1'''
| | </StructureSection> |
| | | [[Category: Large Structures]] |
| | | [[Category: Paramecium bursaria Chlorella virus 1]] |
| ==Overview==
| | [[Category: Rossmann MG]] |
| Paramecium bursaria chlorella virus-1 encodes at least five putative glycosyltransferases that are probably involved in the synthesis of the glycan components of the viral major capsid protein. The 1.6 A crystal structure of one of these glycosyltransferases (A64R) has a mixed alpha/beta fold containing a central, six-stranded beta sheet flanked by alpha helices. Crystal structures of A64R, complexed with UDP, CMP, or GDP, established that only UDP bound to A64R in the presence of Mn(2+), consistent with its high structural similarity to glycosyltransferases which utilize UDP as the sugar carrier. The structure of the complex of A64R, UDP-glucose, and Mn(2+) showed that the largest conformational change occurred when hydrogen bonds were formed with the ligands. Unlike UDP-glucose, UDP-galactose and UDP-GlcNAc did not bind to A64R, suggesting a selective binding of UDP-glucose. Thus, UDP-glucose is most likely the sugar donor for A64R, consistent with glucose occurring in the virus major capsid protein glycans.
| | [[Category: Van Etten JL]] |
| | | [[Category: Xiang Y]] |
| ==About this Structure==
| | [[Category: Zhang Y]] |
| 2P73 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P73 OCA].
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| ==Reference== | |
| Structure and function of a chlorella virus-encoded glycosyltransferase., Zhang Y, Xiang Y, Van Etten JL, Rossmann MG, Structure. 2007 Sep;15(9):1031-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17850743 17850743]
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| [[Category: Paramecium bursaria chlorella virus 1]] | |
| [[Category: Single protein]] | |
| [[Category: Etten, J L.Van.]] | |
| [[Category: Rossmann, M G.]] | |
| [[Category: Xiang, Y.]] | |
| [[Category: Zhang, Y.]] | |
| [[Category: crystal structure]]
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| [[Category: glycosyltransferase]]
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| [[Category: pbcv-1 a64r]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:31:12 2008''
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