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==3.7 angstrom cryoEM structure of truncated mouse TRPM7==
==3.7 angstrom cryoEM structure of truncated mouse TRPM7==
<StructureSection load='6bwd' size='340' side='right' caption='[[6bwd]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
<SX load='6bwd' size='340' side='right' viewer='molstar' caption='[[6bwd]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6bwd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BWD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BWD FirstGlance]. <br>
<table><tr><td colspan='2'>[[6bwd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BWD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BWD FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bwd OCA], [http://pdbe.org/6bwd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bwd RCSB], [http://www.ebi.ac.uk/pdbsum/6bwd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bwd ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bwd OCA], [https://pdbe.org/6bwd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bwd RCSB], [https://www.ebi.ac.uk/pdbsum/6bwd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bwd ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/TRPM7_MOUSE TRPM7_MOUSE] Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).<ref>PMID:11385574</ref>  
The transient receptor potential ion channel subfamily M, member 7 (TRPM7), is a ubiquitously expressed protein that is required for mouse embryonic development. TRPM7 contains both an ion channel and an alpha-kinase. The channel domain comprises a nonselective cation channel with notable permeability to Mg(2+) and Zn(2+) Here, we report the closed state structures of the mouse TRPM7 channel domain in three different ionic conditions to overall resolutions of 3.3, 3.7, and 4.1 A. The structures reveal key residues for an ion binding site in the selectivity filter, with proposed partially hydrated Mg(2+) ions occupying the center of the conduction pore. In high [Mg(2+)], a prominent external disulfide bond is found in the pore helix, which is essential for ion channel function. Our results provide a structural framework for understanding the TRPM1/3/6/7 subfamily and extend the knowledge base upon which to study the diversity and evolution of TRP channels.
 
Structure of the mammalian TRPM7, a magnesium channel required during embryonic development.,Duan J, Li Z, Li J, Hulse RE, Santa-Cruz A, Valinsky WC, Abiria SA, Krapivinsky G, Zhang J, Clapham DE Proc Natl Acad Sci U S A. 2018 Aug 14. pii: 1810719115. doi:, 10.1073/pnas.1810719115. PMID:30108148<ref>PMID:30108148</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6bwd" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Lk3 transgenic mice]]
[[Category: Large Structures]]
[[Category: Abiria, S A]]
[[Category: Mus musculus]]
[[Category: Clapham, D E]]
[[Category: Abiria SA]]
[[Category: Duan, J]]
[[Category: Clapham DE]]
[[Category: Hulse, R E]]
[[Category: Duan J]]
[[Category: Krapivinsky, G]]
[[Category: Hulse RE]]
[[Category: Li, J]]
[[Category: Krapivinsky G]]
[[Category: Li, Z]]
[[Category: Li J]]
[[Category: Santa-Cruz, A]]
[[Category: Li Z]]
[[Category: Zhang, J]]
[[Category: Santa-Cruz A]]
[[Category: Cryoem]]
[[Category: Zhang J]]
[[Category: Membrane protein]]
[[Category: Truncated mouse trpm7]]
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