6cbe: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(3 intermediate revisions by the same user not shown)
Line 1: Line 1:
{{Large structure}}
 
==Atomic structure of a rationally engineered gene delivery vector, AAV2.5==
==Atomic structure of a rationally engineered gene delivery vector, AAV2.5==
<StructureSection load='6cbe' size='340' side='right' caption='[[6cbe]], [[Resolution|resolution]] 2.78&Aring;' scene=''>
<SX load='6cbe' size='340' side='right' viewer='molstar' caption='[[6cbe]], [[Resolution|resolution]] 2.78&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6cbe]] is a 60 chain structure with sequence from [http://en.wikipedia.org/wiki/Aav-2 Aav-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CBE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CBE FirstGlance]. <br>
<table><tr><td colspan='2'>[[6cbe]] is a 60 chain structure with sequence from [https://en.wikipedia.org/wiki/Adeno-associated_virus_2 Adeno-associated virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CBE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CBE FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10804 AAV-2])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.78&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cbe OCA], [http://pdbe.org/6cbe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cbe RCSB], [http://www.ebi.ac.uk/pdbsum/6cbe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cbe ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cbe OCA], [https://pdbe.org/6cbe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cbe RCSB], [https://www.ebi.ac.uk/pdbsum/6cbe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cbe ProSAT]</span></td></tr>
</table>
</table>
{{Large structure}}
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/CAPSD_AAV2S CAPSD_AAV2S]] Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus.<ref>PMID:10684294</ref> <ref>PMID:11961250</ref> <ref>PMID:16940508</ref> <ref>PMID:9445046</ref>
[https://www.uniprot.org/uniprot/CAPSD_AAV2S CAPSD_AAV2S] Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus.<ref>PMID:10684294</ref> <ref>PMID:11961250</ref> <ref>PMID:16940508</ref> <ref>PMID:9445046</ref>  
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
Line 19: Line 18:
</div>
</div>
<div class="pdbe-citations 6cbe" style="background-color:#fffaf0;"></div>
<div class="pdbe-citations 6cbe" style="background-color:#fffaf0;"></div>
==See Also==
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Aav-2]]
[[Category: Adeno-associated virus 2]]
[[Category: Agbandje-McKenna, M]]
[[Category: Large Structures]]
[[Category: Bennett, A]]
[[Category: Agbandje-McKenna M]]
[[Category: Burg, M]]
[[Category: Bennett A]]
[[Category: Byrne, B]]
[[Category: Burg M]]
[[Category: Chipman, P]]
[[Category: Byrne B]]
[[Category: Drouin, L]]
[[Category: Chipman P]]
[[Category: Kozyreva, O G]]
[[Category: Drouin L]]
[[Category: McKenna, R]]
[[Category: Kozyreva OG]]
[[Category: Mietzsch, M]]
[[Category: McKenna R]]
[[Category: Potter, M]]
[[Category: Mietzsch M]]
[[Category: Rosebrough, C]]
[[Category: Potter M]]
[[Category: Samulski, R J]]
[[Category: Rosebrough C]]
[[Category: Sousa, D]]
[[Category: Samulski RJ]]
[[Category: Aav]]
[[Category: Sousa D]]
[[Category: Dependoparvovirus]]
[[Category: Gene therapy vector]]
[[Category: Retional design]]
[[Category: Virus]]
Retrieved from "https://proteopedia.org/6cbe"