6hjx: Difference between revisions

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New page: '''Unreleased structure''' The entry 6hjx is ON HOLD Authors: Spurny, R., Govaerts, C., Evans, G.L., Pardon, E., Steyaert, J., Ulens, C. Description: X-ray structure of a pentameric li...
 
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'''Unreleased structure'''


The entry 6hjx is ON HOLD
==X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) 7'C pore mutant (L238C) in complex with nanobody 72==
<StructureSection load='6hjx' size='340' side='right'caption='[[6hjx]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6hjx]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HJX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PTY:PHOSPHATIDYLETHANOLAMINE'>PTY</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hjx OCA], [https://pdbe.org/6hjx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hjx RCSB], [https://www.ebi.ac.uk/pdbsum/6hjx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hjx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ELIC_DICCH ELIC_DICCH]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Phospholipids are key components of cellular membranes and are emerging as important functional regulators of different membrane proteins, including pentameric ligand-gated ion channels (pLGICs). Here, we take advantage of the prokaryote channel ELIC (Erwinia ligand-gated ion channel) as a model to understand the determinants of phospholipid interactions in this family of receptors. A high-resolution structure of ELIC in a lipid-bound state reveals a phospholipid site at the lower half of pore-forming transmembrane helices M1 and M4 and at a nearby site for neurosteroids, cholesterol or general anesthetics. This site is shaped by an M4-helix kink and a Trp-Arg-Pro triad that is highly conserved in eukaryote GABAA/C and glycine receptors. A combined approach reveals that M4 is intrinsically flexible and that M4 deletions or disruptions of the lipid-binding site accelerate desensitization in ELIC, suggesting that lipid interactions shape the agonist response. Our data offer a structural context for understanding lipid modulation in pLGICs.


Authors: Spurny, R., Govaerts, C., Evans, G.L., Pardon, E., Steyaert, J., Ulens, C.
A lipid site shapes the agonist response of a pentameric ligand-gated ion channel.,Henault CM, Govaerts C, Spurny R, Brams M, Estrada-Mondragon A, Lynch J, Bertrand D, Pardon E, Evans GL, Woods K, Elberson BW, Cuello LG, Brannigan G, Nury H, Steyaert J, Baenziger JE, Ulens C Nat Chem Biol. 2019 Oct 7. pii: 10.1038/s41589-019-0369-4. doi:, 10.1038/s41589-019-0369-4. PMID:31591563<ref>PMID:31591563</ref>


Description: X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) 7'C pore mutant (L238C) in complex with nanobody 72
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Steyaert, J]]
<div class="pdbe-citations 6hjx" style="background-color:#fffaf0;"></div>
[[Category: Govaerts, C]]
 
[[Category: Evans, G.L]]
==See Also==
[[Category: Ulens, C]]
*[[Antibody 3D structures|Antibody 3D structures]]
[[Category: Pardon, E]]
*[[Ion channels 3D structures|Ion channels 3D structures]]
[[Category: Spurny, R]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Dickeya chrysanthemi]]
[[Category: Lama glama]]
[[Category: Large Structures]]
[[Category: Evans GL]]
[[Category: Govaerts C]]
[[Category: Pardon E]]
[[Category: Spurny R]]
[[Category: Steyaert J]]
[[Category: Ulens C]]
Retrieved from "https://proteopedia.org/6hjx"