2pz8: Difference between revisions

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[[Image:2pz8.jpg|left|200px]]


{{Structure
==NAD+ Synthetase from Bacillus anthracis with AMP-CPP and Mg2+==
|PDB= 2pz8 |SIZE=350|CAPTION= <scene name='initialview01'>2pz8</scene>, resolution 2.00&Aring;
<StructureSection load='2pz8' size='340' side='right'caption='[[2pz8]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
<table><tr><td colspan='2'>[[2pz8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PZ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PZ8 FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)_synthase NAD(+) synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.5 6.3.1.5] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
|GENE= nadE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pz8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pz8 OCA], [https://pdbe.org/2pz8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pz8 RCSB], [https://www.ebi.ac.uk/pdbsum/2pz8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pz8 ProSAT]</span></td></tr>
|RELATEDENTRY=[[2pza|2PZA]], [[2pzb|2PZB]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pz8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pz8 OCA], [http://www.ebi.ac.uk/pdbsum/2pz8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pz8 RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/NADE_BACAN NADE_BACAN]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pz/2pz8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pz8 ConSurf].
<div style="clear:both"></div>


'''NAD+ Synthetase from Bacillus anthracis with AMP-CPP and Mg2+'''
==See Also==
 
*[[NAD synthase|NAD synthase]]
 
__TOC__
==Overview==
</StructureSection>
The crystal structures of NH(3)-dependent NAD+ synthetase from Bacillus anthracis as the apoenzyme (1.9 A), in complex with the natural catalytic products AMP and pyrophosphate (2.4 A) and in complex with the substrate analog adenosine 5'-(alpha,beta-methylene)triphosphate (2.0 A) have been determined. NAD+ synthetase catalyzes the last step in the biosynthesis of the vitally important cofactor NAD+. In comparison to other NAD+ synthetase crystal structures, the C-terminal His-tagged end of the apoenzyme adopts a novel helical conformation, causing significant compensatory changes in the region. The structural accommodations observed in B. anthracis NAD+ synthetase are remarkable in the absence of adverse affects on enzyme activity. They also illustrate a rare example of the influence of a non-native C-terminal His-tag extension on the structure of a native protein. In contrast to the apoenzyme, when AMP and pyrophosphate or adenosine 5'-(alpha,beta-methylene)triphosphate are bound, the C-terminus adopts a conformation that allows ATP binding and overall the structure then resembles other NAD+ synthetase structures. The structures of NAD+ synthetase complexes from B. anthracis are compared with published X-ray crystal structures of the enzyme from B. subtilis, Escherichia coli and Helicobacter pylori. These comparisons support the novel observation that P1 and P2 loop ordering is not a consequence of crystal contacts but rather a consequence of intrinsic intramolecular interactions within the ordered subunit.
 
==About this Structure==
2PZ8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PZ8 OCA].
 
==Reference==
Structural adaptation of an interacting non-native C-terminal helical extension revealed in the crystal structure of NAD+ synthetase from Bacillus anthracis., McDonald HM, Pruett PS, Deivanayagam C, Protasevich II, Carson WM, DeLucas LJ, Brouillette WJ, Brouillette CG, Acta Crystallogr D Biol Crystallogr. 2007 Aug;63(Pt 8):891-905. Epub 2007, Jul 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17642516 17642516]
[[Category: Bacillus anthracis]]
[[Category: Bacillus anthracis]]
[[Category: NAD(+) synthase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Brouillette CG]]
[[Category: Brouillette, C G.]]
[[Category: Brouillette WJ]]
[[Category: Brouillette, W J.]]
[[Category: Carson WM]]
[[Category: Carson, W M.]]
[[Category: DeLucas LJ]]
[[Category: DeLucas, L J.]]
[[Category: Deivanayagam C]]
[[Category: Deivanayagam, C.]]
[[Category: McDonald HM]]
[[Category: McDonald, H M.]]
[[Category: Protasevich II]]
[[Category: Protasevich, I I.]]
[[Category: Pruett PS]]
[[Category: Pruett, P S.]]
[[Category: his-tag]]
[[Category: ligase]]
[[Category: protein-substrate analog complex]]
 
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Retrieved from "https://proteopedia.org/2pz8"