6a2v

Crystal structure of Hcp protein

spinqualitylabelsall models 6a2v, resolution 2.59Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6a2v is a 6 chain structure with sequence from Campylobacter jejuni subsp. jejuni. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.588Å Ligands: MSE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The Type VI Secretion System (T6SS) provides enhanced virulence to Campylobacter jejuni and has been associated with a high incidence of bloody diarrhea. The hemolysin-coregulated protein (Hcp)-the hallmark of the T6SS-can act as a structural and effector protein. Unlike other T6SS-harboring bacteria, which possess multiple Hcp proteins each performing different functions, C. jejuni possesses only one Hcp protein, and its structural and functional role has not been elucidated previously. Here, we report the structure and functional studies of Hcp from C. jejuni. We found similarities between the hexameric ring structure of Hcp-Cj and that of Hcp3 from Pseudomonas aeruginosa. Through functional studies, we showed two roles for Hcp-Cj i.e., in cytotoxicity towards HepG2 cells and in biofilm formation in C. jejuni. In structure-based mutational analyses, we showed that an Arg-to-Ala mutation at position 30 within the extended loop results in a significant decrease in cytotoxicity, suggesting a role for this loop in binding to the host cell. However, this mutation does not affect its biofilm formation function. Collectively, this study supports the dual role of Hcp-Cj as a structural and effector protein in C. jejuni. This article is protected by copyright. All rights reserved. Structural basis for the pathogenesis of Campylobacter jejuni Hcp1, a structural and effector protein of the type six secretion system.,Noreen Z, Jobichen C, Abbasi R, Seetharaman J, Sivaraman J, Bohkhari H FEBS J. 2018 Sep 7. doi: 10.1111/febs.14650. PMID:30194714 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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