2w14: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,329,557 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,329,557 edits
No edit summary
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:


==High-resolution crystal structure of the P-I snake venom metalloproteinase BaP1 in complex with a peptidomimetic: insights into inhibitor binding==
==High-resolution crystal structure of the P-I snake venom metalloproteinase BaP1 in complex with a peptidomimetic: insights into inhibitor binding==
<StructureSection load='2w14' size='340' side='right' caption='[[2w14]], [[Resolution|resolution]] 1.08&Aring;' scene=''>
<StructureSection load='2w14' size='340' side='right'caption='[[2w14]], [[Resolution|resolution]] 1.08&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2w14]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bothrops_asper Bothrops asper]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W14 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W14 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2w14]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bothrops_asper Bothrops asper]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W14 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W14 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=WR2:(2R,3R)-N^1^-[(1S)-2,2-DIMETHYL-1-(METHYLCARBAMOYL)PROPYL]-N^4^-HYDROXY-2-(2-METHYLPROPYL)-3-{[(1,3-THIAZOL-2-YLCARBONYL)AMINO]METHYL}BUTANEDIAMIDE'>WR2</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.08&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=WR2:(2R,3R)-N^1^-[(1S)-2,2-DIMETHYL-1-(METHYLCARBAMOYL)PROPYL]-N^4^-HYDROXY-2-(2-METHYLPROPYL)-3-{[(1,3-THIAZOL-2-YLCARBONYL)AMINO]METHYL}BUTANEDIAMIDE'>WR2</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2w15|2w15]], [[1nd1|1nd1]], [[2w13|2w13]], [[2w12|2w12]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w14 OCA], [https://pdbe.org/2w14 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w14 RCSB], [https://www.ebi.ac.uk/pdbsum/2w14 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w14 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w14 OCA], [http://pdbe.org/2w14 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w14 RCSB], [http://www.ebi.ac.uk/pdbsum/2w14 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w14 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/VM1B1_BOTAS VM1B1_BOTAS]] Zinc metalloprotease that exhibits a weak hemorrhagic activity (with a minimum hemorrhagic dose of 20 ug by intradermal and intramuscular injection into mice). The basal membrane components collagen (all chains of type IV) (COL4A4), laminin and nidogen are all degraded by this toxin (PubMed:23385358). Rapidly degrades the Aalpha-chain (FGA) of fibrinogen, and later on, degrades the Bbeta-chain (FGB) of fibrinogen (PubMed:7778126). Also activates the complement system, and induces rat neutrophil chemotaxis (PubMed:11200361). Induces edema in mouse food pad and a mild myotoxicity (PubMed:7778126).<ref>PMID:11200361</ref> <ref>PMID:20600221</ref> <ref>PMID:23385358</ref> <ref>PMID:7778126</ref>
[https://www.uniprot.org/uniprot/VM1B1_BOTAS VM1B1_BOTAS] Zinc metalloprotease that exhibits a weak hemorrhagic activity (with a minimum hemorrhagic dose of 20 ug by intradermal and intramuscular injection into mice). The basal membrane components collagen (all chains of type IV) (COL4A4), laminin and nidogen are all degraded by this toxin (PubMed:23385358). Rapidly degrades the Aalpha-chain (FGA) of fibrinogen, and later on, degrades the Bbeta-chain (FGB) of fibrinogen (PubMed:7778126). Also activates the complement system, and induces rat neutrophil chemotaxis (PubMed:11200361). Induces edema in mouse food pad and a mild myotoxicity (PubMed:7778126).<ref>PMID:11200361</ref> <ref>PMID:20600221</ref> <ref>PMID:23385358</ref> <ref>PMID:7778126</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Line 35: Line 34:
</StructureSection>
</StructureSection>
[[Category: Bothrops asper]]
[[Category: Bothrops asper]]
[[Category: Gutierrez, J M]]
[[Category: Large Structures]]
[[Category: Lingott, T J]]
[[Category: Gutierrez JM]]
[[Category: Merfort, I]]
[[Category: Lingott TJ]]
[[Category: Schleberger, C]]
[[Category: Merfort I]]
[[Category: Adamalysin]]
[[Category: Schleberger C]]
[[Category: Alpha-beta protein]]
[[Category: Chemotaxis]]
[[Category: Endopeptidase]]
[[Category: Hydrolase]]
[[Category: Hydrolase inhibitor complex]]
[[Category: Hydrolase-inhibitor complex]]
[[Category: Matrixmetalloproteinase]]
[[Category: Metal-binding]]
[[Category: Metalloprotease]]
[[Category: Metalloproteinase-inhibitor complex]]
[[Category: Metzincin]]
[[Category: P-i snake venom metalloproteinase]]
[[Category: Protease]]
[[Category: Pyrrolidone carboxylic acid]]
[[Category: Secreted]]
[[Category: Toxin]]
[[Category: Tumor necrosis factor alpha converting enzyme]]
[[Category: Zinc]]
[[Category: Zinc-depending]]
Retrieved from "https://proteopedia.org/2w14"